Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice
| Autor: | Yukiko Tomioka, Yuki Watanabe, Etsuro Ono, Minako Kuramochi, Keiko Amagai, Toshio Wada, Shigeto Kouda, Masami Morimatsu, Noritaka Kuboki |
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| Rok vydání: | 2009 |
| Předmět: |
Genetically modified mouse
Transgene Recombinant Fusion Proteins Immunology Nectins Sus scrofa Biology Microbiology Chimera (genetics) Mice Nectin Virology Animals Humans Receptor Cells Cultured Pseudorabies Fibroblasts Molecular biology Fusion protein Herpesvirus 1 Suid Immunity Innate Immunoglobulin Fc Fragments Protein Structure Tertiary Mice Inbred C57BL Ectodomain biology.protein Antibody Cell Adhesion Molecules |
| Zdroj: | Microbiology and immunology. 53(1) |
| ISSN: | 0385-5600 |
| Popis: | Nectin-1 is a Ca2+-independent Ig-like cell–cell adhesion molecule and an alphaherpesvirus receptor that binds to virion glycoprotein D by the first Ig-like domain. We have investigated the antiviral potentials of soluble forms of porcine nectin-1 to PRV infection by generating transgenic mice expressing different types of fusion protein. Previously, we reported that mice transgenic for a chimera that carried the entire ectodomain of porcine nectin-1 fused to the Fc portion of porcine IgG1 were more resistant than those transgenic for a chimera that carried the first Ig-like domain fused to the Fc portion. Recently, we generated transgenic mice expressing a fusion protein made of the first Ig-like domain fused to the Fc portion of human IgG1, and reported that they showed a microphthalmia. Here, two transgenic mouse lines expressing the fusion protein were challenged with PRV for comparing their resistances with those of transgenic mice expressing different types of fusion protein. Surprisingly, both transgenic mouse lines showed a high resistance to the viral infection, especially via the i.n. route. Significant resistance of the embryonic fibroblasts was also observed. Altogether, these findings indicated that the fusion protein consisting of the first Ig-like domain fused to the human Fc portion provided a marked resistance against PRV infection to the transgenic mice. |
| Databáze: | OpenAIRE |
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