EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS
| Autor: | CAMPOS, Letícia Fuganti, TAGLIARI, Eliane, CASAGRANDE, Thais Andrade Costa, de NORONHA, Lúcia, CAMPOS, Antônio Carlos L., MATIAS, Jorge Eduardo F |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Contraction (grammar) Skin wound RD1-811 Wound healing RC799-869 Diabetes Mellitus Experimental law.invention 03 medical and health sciences chemistry.chemical_compound Probiotic 0302 clinical medicine Diabetes mellitus law Alloxan Diabete melito Animals Humans Medicine Rats Wistar Aloxano business.industry Probiotics Probióticos General Medicine Diseases of the digestive system. Gastroenterology medicine.disease Rats 030104 developmental biology chemistry 030220 oncology & carcinogenesis Anesthesia Original Article Surgery Cicatrização business |
| Zdroj: | ABCD: Arquivos Brasileiros de Cirurgia Digestiva, Vol 33, Iss 1 (2020) Arquivos Brasileiros de Cirurgia Digestiva : ABCD ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) v.33 n.1 2020 ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) Colégio Brasileiro de Cirurgia Digestiva (CBCD) instacron:CBCD ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), Volume: 33, Issue: 1, Article number: e1498, Published: 08 JUL 2020 |
| ISSN: | 0102-6720 |
| Popis: | Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p Graphical Abstract Example of wound contraction area at: A) day of surgery in the control group; B) 7PO in the control group; C) day of surgery in the probiotic group; D) 7PO in probiotic group |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|