High throughput microRNAs sequencing profile of serum exosomes in women with and without polycystic ovarian syndrome
| Autor: | Fei-Jun Ye, Yao He, Hai-Tao Pan, Juan Zhang, Feng Zhang, Su-Ping Li, Ying-Ying Ma, Hua Yuan, Tao Zhang, Hai-Gang Ding, Bin Yu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serum
endocrine system diseases Women’s Health lcsh:Medicine Differentially expressed mirnas Bioinformatics Exosomes General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine microRNA PCOS Endocrine system Medicine Gynecology and Obstetrics Molecular Biology 030304 developmental biology 0303 health sciences business.industry General Neuroscience lcsh:R General Medicine Genomics Biomarker Polycystic ovary Microvesicles female genital diseases and pregnancy complications microRNAs 030220 oncology & carcinogenesis Potential biomarkers Clinical diagnosis Biomarker (medicine) General Agricultural and Biological Sciences business |
| Zdroj: | PeerJ, Vol 9, p e10998 (2021) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | Background Polycystic ovary syndrome (PCOS) is the most common type of endocrine disorder, affecting 5–11% of women of reproductive age worldwide. microRNAs (miRNAs) stably exist in circulating blood encapsulated in extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve as novel PCOS biomarkers. Methods To identify miRNA biomarkers that are associated with PCOS, we performed a comprehensive sequence-based characterization of the PCOS serum miRNA landscape. The serum exosomes were successfully isolated and characterized in a variety of ways. Next, sequence-based analysis was performed on serum exosomes to screen the differentially expressed miRNAs in women with and without PCOS. Results The sequence data revealed that the levels of 54 miRNAs significantly differed between PCOS patients and normal controls. The levels of these miRNAs were detected by RT-qPCR. The results show that hsa-miR-1299, hsa-miR-6818-5p hsa-miR-192-5p, and hsa-miR-145-5p are significantly differentially expressed in PCOS patients serum exosomes and identify these microRNAs as potential biomarkers for PCOS. Furthermore, Gene Ontology (GO) analyses and KEGG pathway analyses of the miRNA targets further allowed to explore the potential implication of the miRNAs in PCOS. Conclusion Our findings suggest that serum exosomal miRNAs serve important roles in PCOS and may be used as novel molecular biomarkers for clinical diagnosis. |
| Databáze: | OpenAIRE |
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