Searching for Biological Function of the Mysterious PA2504 Protein from Pseudomonas aeruginosa
| Autor: | Kamil Steczkiewicz, Elzbieta Kraszewska, Martyna Kujawa, Joanna Drabinska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
PA2504 RppH Biology Catalysis Inorganic Chemistry Transcriptome Nudix Ribosomal protein Transcriptional regulation crosslink in vivo RNA-Seq Physical and Theoretical Chemistry Biology (General) Molecular Biology Gene QD1-999 Spectroscopy Organic Chemistry RNA General Medicine Computer Science Applications Cell biology TUDOR domain Pseudomonas aeruginosa Response regulator Chemistry Proteome sulphur metabolism Function (biology) |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 9833, p 9833 (2021) International Journal of Molecular Sciences Volume 22 Issue 18 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | For nearly half of the proteome of an important pathogen, Pseudomonas aeruginosa, the function has not yet been recognised. Here, we characterise one such mysterious protein PA2504, originally isolated by us as a sole partner of the RppH RNA hydrolase involved in transcription regulation of multiple genes. This study aims at elucidating details of PA2504 function and discussing its implications for bacterial biology. We show that PA2504 forms homodimers and is evenly distributed in the cytoplasm of bacterial cells. Molecular modelling identified the presence of a Tudor-like domain in PA2504. Transcriptomic analysis of a ΔPA2504 mutant showed that 42 transcripts, mainly coding for proteins involved in sulphur metabolism, were affected by the lack of PA2504. In vivo crosslinking of cellular proteins in the exponential and stationary phase of growth revealed several polypeptides that bound to PA2504 exclusively in the stationary phase. Mass spectrometry analysis identified them as the 30S ribosomal protein S4, the translation elongation factor TufA, and the global response regulator GacA. These results indicate that PA2504 may function as a tether for several important cellular factors. |
| Databáze: | OpenAIRE |
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