Even Attenuated Bovine Leukemia Virus Proviruses Can Be Pathogenic in Sheep
| Autor: | Nicolas Gillet, Mathieu Boxus, Richard Kettmann, Lucas Willems, Pierre Kerkhofs, Arnaud Florins |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Lymphoma Amino Acid Motifs DNA Mutational Analysis Immunology Mutant Sheep Diseases Biology Microbiology Virus Viral Proteins Virology Virus latency Leukemia Virus Bovine medicine Animals Sequence Deletion Sheep Bovine leukemia virus Wild type Membrane Proteins Enzootic Bovine Leukosis Provirus Cell Transformation Viral medicine.disease biology.organism_classification Reverse genetics Virus Latency Leukemia Insect Science Pathogenesis and Immunity Cattle |
| Zdroj: | Journal of Virology. 81:10195-10200 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Based on a reverse genetics approach, we previously reported that bovine leukemia virus (BLV) mutants harboring deletions in the accessory R3 and G4 genes persist at very low proviral loads and are unable to induce leukemia or lymphoma in sheep, indicating that these R3 and G4 gene sequences are required for pathogenesis. We now show that lymphoma can occur, albeit infrequently (1 case of 20) and after extended periods of latency (7 years). Direct sequencing and reinfection experiments demonstrated that lymphomagenesis was not due to the reversion of the mutant to the wild type. Similar observations with another type of attenuated mutant impaired in the transmembrane protein (TM) YXXL signaling motifs were made. We conclude that the R3 and G4 genes and the TM YXXL motifs are not strictly required for pathogenesis but that their integrity contributes to disease frequency and latency. |
| Databáze: | OpenAIRE |
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