Morphological change of the cupula due to an ototoxic agent: A comparison with semicircular canal pathology
| Autor: | Go Hasegawa, Rei Motohashi, Koji Otsuka, Taro Inagaki, Ujimoto Konomi, Shigetaka Shimizu, Mamoru Suzuki, Akira Shimizu |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Sensory system Rana Ototoxicity medicine Animals Inner ear skin and connective tissue diseases Saline Rana catesbeiana Semicircular canal Chemistry Posterior Semicircular Canal Hair Cells Ampulla Semicircular Ducts General Medicine Anatomy medicine.disease Semicircular Canals Anti-Bacterial Agents medicine.anatomical_structure Otorhinolaryngology Ampullae of Lorenzini Microscopy Electron Scanning sense organs Gentamicins |
| Zdroj: | Acta Oto-Laryngologica. 130:652-658 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.3109/00016480903370779 |
| Popis: | The cupula shows various degrees of changes after gentamicin (GM) injection into the inner ear, with or without damage of the sensory cells. This cupula change may be a part of the etiology of peripheral vertigo, and is also potentially one of the mechanisms of reduced caloric response.To observe the morphological changes of the cupula after injecting GM in the frog inner ear and to compare the changes of the cupula with those of the ampullary sensory cells.We injected 300 microg (7.5 microl) of GM into the inner ear of 30 bullfrogs (Rana catesbeiana) using a microsyringe under ether anesthesia. The same amount of saline was injected into the other ear as control. The cupulae were observed at 3, 7, and 14 days after GM injection by stereoscopic microscope. The ampullae were fixed, and the sensory cells were assessed using a scanning electron microscope (SEM). The correlation between the changes in the cupula and sensory cells was evaluated using our own scale.In over half of the cupulae in the 7- and 14-day groups, cupula changes such as shrinkage were observed. In about 50% of the total cases, the degree of cupula and sensory cell change correlated in the two groups. In the 14-day group, these changes were more marked. However, there were cases in which the changes of the cupula and sensory cells did not correlate, indicating that the cupula alone can sustain changes without sensory cell damage. |
| Databáze: | OpenAIRE |
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