Effects of quercetin on Aroclor 1254-induced expression of CYP450 and cytokines in pregnant rats

Autor:	Yu Zhong Ma, Xiaojun Guo, Nan Li, Lina Xu, Qing Pan
Rok vydání:	2019
Předmět:	lcsh:Immunologic diseases. Allergy ifnγ/il-4 aroclor 1254 Immunology embryo 010501 environmental sciences liver Toxicology 01 natural sciences quercetin Andrology 03 medical and health sciences chemistry.chemical_compound lcsh:RA1190-1270 rat heterocyclic compounds reproductive and urinary physiology lcsh:Toxicology. Poisons 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Chemistry organic chemicals Embryo cytokines embryonic structures lcsh:RC581-607 Quercetin cyp450
Zdroj:	Journal of Immunotoxicology, Vol 16, Iss 1, Pp 140-148 (2019)
ISSN:	1547-6901 1547-691X
DOI:	10.1080/1547691x.2019.1604585
Popis:	The present study aimed to investigate the protective effect of quercetin on polychlorinated biphenyls (PCB)-induced liver and embryo damage in pregnant Sprague–Dawley rats. Pregnant rats were divided into five groups, and then were orally gavaged daily with peanut oil (vehicle) or a commercial PCB mixture (Aroclor 1254) — with or without co-treatment with 75, 150, or 300 mg/kg quercetin — on gestation days (GD) 4–7. At GD 9, all rats were euthanized, and their blood, liver, and uterus were collected. Expressions of CYP450 mRNA and protein in liver, cytokines (IFNγ, IL-2, IL-4, and IL-6) and IFNγ/IL-4 ratios in liver and sera, liver morphology, and the status of implanted embryos were analyzed. The results showed Aroclor 1254 treatment alone caused hepatic cord damage (i.e. cell disorganization, swelling, decreased cytoplasm, vacuolization), and that quercetin co-treatment appeared to mitigate this damage. Similarly, levels of CYP1A1 and CYP2B1 mRNA in livers of Aroclor 1254-only-treated rats were significantly higher than those in rats co-treated with quercetin. Hepatic and sera levels of IFNγ, IL-2, IL-6, and IFNγ/IL-4 ratios, and the ratio of delayed-development embryos, all increased in Aroclor 1254-treated rats, but were relatively decreased as a result of quercetin co-treatments. IL-4 levels were decreased by Aroclor 1254 and tended to increase back to normal when quercetin was used. The results indicated that quercetin imparted a protective effect against Aroclor 1254-induced toxicity in pregnant rats, in part, by modulating levels of important pro-inflammatory cytokines and reducing induced CYP1A1 and CYP2B1 expression.
Databáze:	OpenAIRE
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