Growth differentiation factor 11 inhibits adipogenic differentiation by activating TGF‐beta/Smad signalling pathway
| Autor: | Yu Ban, Lin Peng, Yuchen Guo, Yuan Wang, Rixin Zheng, Yuting Liu, Hongke Luo, Quan Yuan, Weiqing Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
3T3‐L1 pre‐adipocytes
0301 basic medicine growth differentiation factor 11 Gene Expression Smad Proteins SMAD Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transforming Growth Factor beta 3T3-L1 Cells TGF beta signaling pathway Gene expression Adipocytes TGF‐beta Animals Humans Oil Red O mesenchymal stem cells Adipogenesis Mesenchymal stem cell Cell Differentiation Original Articles Cell Biology General Medicine Hedgehog signaling pathway Cell biology Growth Differentiation Factors 030104 developmental biology chemistry 030220 oncology & carcinogenesis Bone Morphogenetic Proteins GDF11 Original Article Signal Transduction |
| Zdroj: | Cell Proliferation |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/cpr.12631 |
| Popis: | Objectives Growth differentiation factor 11 (GDF11), an emerging secreted member of the TGF‐beta superfamily, plays essential roles in development, physiology and multiple diseases; however, its role during adipogenic differentiation and the underlying mechanisms remains poorly understood. Materials and methods Bone marrow‐derived human mesenchymal stem cells (hMSCs) and 3T3‐L1 pre‐adipocytes were induced with adipogenic culture medium supplementing with different concentrations of recombinant GDF11 (rGDF11 0, 10, 50, 100 ng mL−1). Oil Red O staining, qRT‐PCR analysis, Western blot analysis and immunofluorescence staining were performed to assay adipogenesis. Results For both hMSCs and 3T3‐L1 pre‐adipocytes, the presence of rGDF11 leads to a dose‐dependent reduction of intracellular lipid droplet accumulation and suppressed adipogenic‐related gene expression. Mechanically, GDF11 inhibits adipogenesis by activating Smad2/3‐dependent TGF‐beta signalling pathway, and these inhibitory effects could be restored by SB‐431542, a pharmacological TGF‐beta type I receptor inhibitor. Conclusions Taken together, our data indicates that GDF11 inhibits adipogenic differentiation in both hMSCs and 3T3‐L1 pre‐adipocytes by activating Smad2/3‐dependent TGF‐beta signalling pathway. |
| Databáze: | OpenAIRE |
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