Strategy using a new antigenic test for rapid diagnosis of Streptococcus pneumoniae infection in respiratory samples from children consulting at hospital

Autor:	E. Begaud, Cyrille H. Haddar, Aymeric Cantais, Johan Md Joly, Yves Germani, Paul O. Verhoeven, O. Mory, Florence Grattard, Bruno Pozzetto, Anne Carricajo
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Microbiology (medical) Male Microbiological Techniques Adolescent PCR assay lcsh:QR1-502 Rapid diagnostic test Biology medicine.disease_cause Microbiology Sensitivity and Specificity Pneumococcal Infections lcsh:Microbiology 03 medical and health sciences 0302 clinical medicine Respiratory infection Streptococcus pneumoniae parasitic diseases medicine Humans 030212 general & internal medicine Child Respiratory Tract Infections 0303 health sciences Antigens Bacterial 030306 microbiology Polysaccharides Bacterial Bacterial pneumonia Infant Newborn Infant Pneumonia medicine.disease Prognosis respiratory tract diseases Vaccination Pneumococcal infections Early Diagnosis Parasitology Child Preschool Female Research Article
Zdroj:	BMC Microbiology, Vol 20, Iss 1, Pp 1-9 (2020) BMC Microbiology
ISSN:	1471-2180
DOI:	10.1186/s12866-020-01764-0
Popis:	Background Despite vaccination programs, Streptococcus pneumoniae remains among the main microorganisms involved in bacterial pneumonia, notably in terms of severity. The prognosis of pneumococcal infections is conditioned in part by the precocity of the diagnosis. The aim of this study was to evaluate the impact of a Rapid Diagnostic Test (RDT) targeting cell wall polysaccharide of Streptococcus pneumoniae and performed directly in respiratory samples, on the strategy of diagnosis of respiratory pneumococcal infections in children. Results Upper-respiratory tract samples from 196 children consulting at hospital for respiratory infection were tested for detecting S. pneumoniae using a newly-designed RDT (PneumoResp, Biospeedia), a semi-quantitative culture and two PCR assays. If positive on fluidized undiluted specimen, the RDT was repeated on 1:100-diluted sample. The RDT was found highly specific when tested on non-S. pneumoniae strains. By comparison to culture and PCR assays, the RDT on undiluted secretions exhibited a sensitivity (Se) and negative predictive value (NPV) of more than 98%. By comparison to criteria of S. pneumoniae pneumonia combining typical symptoms, X-ray image, and culture ≥107 CFU/ml, the Se and NPV of RDT on diluted specimens were 100% in both cases. Conclusions In case of negative result, the excellent NPV of RDT on undiluted secretions allows excluding S. pneumoniae pneumonia. In case of positive result, the excellent sensitivity of RDT on diluted secretions for the diagnosis of S. pneumoniae pneumonia allows proposing a suitable antimicrobial treatment at day 0.
Databáze:	OpenAIRE
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