Inhibition of hepatitis A virus replication in vitro by antiviral compounds
| Autor: | Jacques Passagot, Evangelos Biziagos, Jean-Marc Crance, H. van Cuyck-Gandré, R Deloince |
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| Rok vydání: | 1990 |
| Předmět: |
Carcinoma
Hepatocellular viruses Drug Evaluation Preclinical Pyrazofurin Hepatitis A Antigens Biology Virus Replication Antiviral Agents Virus chemistry.chemical_compound Antigen Virology Tumor Cells Cultured medicine Humans Hepatovirus Glycyrrhizin Antigens Viral Ribavirin Liver Neoplasms Amantadine virus diseases Effective dose (pharmacology) digestive system diseases Infectious Diseases chemistry Depression Chemical medicine.drug |
| Zdroj: | Journal of Medical Virology. 31:155-160 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.1890310214 |
| Popis: | Forty antiviral compounds were screened for inhibitory effect on hepatitis A virus (HAV) antigen expression in the human hepatoma cell line PLC/PRF/5. Ribavirin, amantadine, glycyrrhizin, and pyrazofurin were selected in this screening test and were studied further. The selectivity indices of these four compounds, calculated as the ratio of 50% cytotoxic dose (determined by the trypan blue exclusion and by inhibition of [3H] leucine incorporation) to the 50% effective dose (determined by the viral antigen expression), were 4.6 and 3.0 with ribavirin, 5.3 and 5.9 with amantadine, 15.2 and 16.9 with glycyrrhizin, and 45.4 and 74.6 with pyrazofurin. All four compounds resulted in concentration-dependent reductions of HAV antigen expression and HAV infectivity. Ribavirin, amantadine, pyrazofurin, and glycyrrhizin emerged, from the present study, as promising candidates for chemotherapy of acute hepatitis A. |
| Databáze: | OpenAIRE |
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