Cystic Canal Mutants in Caenorhabditis elegans Are Defective in the Apical Membrane Domain of the Renal (Excretory) Cell
| Autor: | Edward M. Hedgecock, David H. Hall, Matthew Buechner, Harshida Bhatt |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
nematode
Cell Kidney excretory canals 03 medical and health sciences 0302 clinical medicine Polycystic kidney disease medicine Animals Molecular Biology Cells Cultured Caenorhabditis elegans 030304 developmental biology 0303 health sciences polycystic kidney disease renal epithelium biology Epidermis (botany) Cell Membrane Genetic Complementation Test Chromosome Mapping Cell Differentiation Epithelial Cells Cell Biology Apical membrane medicine.disease biology.organism_classification tubulogenesis Phenotype Cell biology medicine.anatomical_structure Epidermal Cells Excretory system Mutation Caenorhabditis Ultrastructure Epidermis 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 214:227-241 |
| ISSN: | 0012-1606 |
| Popis: | The excretory cell extends a tubular process, or canal, along the basolateral surface of the epidermis to form the nematode renal epithelium. This cell can undergo normal tubulogenesis in isolated cell culture. Mutations in 12 genes cause excretory canal cysts in Caenorhabditis elegans. Genetic interactions, and their similar phenotypes, suggest these genes may encode functionally related proteins. Depending upon genotype and individual canal, defects range from focal cysts, flanked by normal width segments, to regional cysts involving the entire tubule. Oftentimes the enlarged regions are convoluted or partially septated. In mutants with very large cysts, renal function is measurably impaired. Based on histology and ultrastructure, canal cysts likely result from defects of the apical membrane domain. These mutants provide a model of tubulocystic disease without hyperplasia or basement membrane abnormalities. Similar apical mechanisms could regulate tubular morphology of vertebrate nephrons. |
| Databáze: | OpenAIRE |
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