The Search for Fc Receptors on Human Tissues and Human Endothelial Cells in Culture
| Autor: | E. R. Hurd, Y. Hashimoto, Alice R. Johnson, M. Shingu |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium Placenta Antigen-Antibody Complex Receptors Fc Biology Umbilical cord General Biochemistry Genetics and Molecular Biology Umbilical Cord Immune system Phagocytosis Pregnancy medicine Humans Receptor Lung Opsonin Cells Cultured Latex beads Molecular biology medicine.anatomical_structure biology.protein Blood Vessels Female Antibody |
| Zdroj: | Experimental Biology and Medicine. 167:147-155 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-167-41140 |
| Popis: | Human endothelial cells in culture and in tissue sections were examined for Fc receptors by several different methods, including the binding of FITC- and ferritin-labeled antibodies to IgG, rosette formation with sensitized sheep erythrocytes, and binding of opsonized latex particles. Although human polymorphonuclear leukocytes reacted positively in all of these tests, endothelial cells freshly isolated or cultured from umbilical cord veins were consistently negative. A small number of cells cultured from umbilical arteries formed rosettes with sheep erythrocytes, but cells from pulmonary arteries did not. Fc receptors could not be found in vessels of the umbilical cord or in lung tissue sections, but vessels in the placenta contained prominent Fc receptors. Cultured endothelial cells that were suspended by trypsin took up latex beads and immune complexes by endocytosis. Undisturbed monolayers of cells were much less active. This finding suggests that the endothelium may be stimulated by proteolyt... |
| Databáze: | OpenAIRE |
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