In vitro prothrombin synthesis from a purified precursor protein
| Autor: | Berry A.M. Soute, Cees Vermeer, H.C. Hemker |
|---|---|
| Přispěvatelé: | Biochemie |
| Jazyk: | angličtina |
| Rok vydání: | 1978 |
| Předmět: |
chemistry.chemical_classification
Vitamin Vitamin K Carboxy-Lyases Warfarin General Medicine Factor ii In vitro Pyruvate carboxylase Oxygen Kinetics chemistry.chemical_compound Enzyme chemistry Biochemistry Enzyme system Factor II Activity Microsomes Liver medicine Animals Cattle Female Prothrombin medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA)-Enzymology, 523(2), 494-505. Elsevier |
| ISSN: | 0005-2744 |
| DOI: | 10.1016/0005-2744(78)90052-9 |
| Popis: | In this paper, we describe the isolation and partial purification of an enzyme system that converts bovine decarboxyfactor II (PIVKA-II) into prothrombin (factor II). It is shown that the increase in factor II activity occurs in parallel with 14CO2 incorporation into BaSO4 adsorbable proteins. The system is not strictly vitamin K-dependent because it is obtained from the livers of normal healthy cows. By preincubating the enzyme(s) with an excess of warfarin, an absolute vitamin K1-dependence can be obtained. The reaction is inhibited by its own product, factor II. |
| Databáze: | OpenAIRE |
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