The preparation of D-myo-inositol 1,4-bisphosphate and D-myo-inositol 1,4,5-trisphosphate in milligram quantities from a readily available starting material
| Autor: | I. M. Bird, I.H. Sadler, S W Walker, Brent C. Williams |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Reaction mechanism Chromatography Magnetic Resonance Spectroscopy Time Factors Dose-Response Relationship Drug Inositol Phosphates Periodic Acid Periodate High resolution Inositol 1 4 5-Trisphosphate Biochemistry Phosphates chemistry.chemical_compound Endocrinology Column chromatography chemistry Yield (chemistry) Inositol Spectrophotometry Ultraviolet Chromatography Thin Layer Inositol phosphate Molecular Biology MYO INOSITOL 1 4 5 TRISPHOSPHATE |
| Zdroj: | Molecular and cellular endocrinology. 66(2) |
| ISSN: | 0303-7207 |
| Popis: | The optimisation of a reaction for the conversion of glycerophosphoinositols to phosphoinositols is described. This reaction has been used in a scheme, described in detail, for the formation of d -myo-inositol 1,4-bisphosphate and d -myo-inositol 1,4,5-trisphosphate in mg quantities from a readily available preparation of mixed phosphoinositides. An optimised procedure is also detailed for the recovery of these products to high yield and purity. The identity of the products has been confirmed both by high resolution anion-exchange column chromatography and by 1H nuclear magnetic resonance studies. We report for the first time the 1H nuclear magnetic resonance spectrum for d -myo-inositol 1,4-bisphosphate. |
| Databáze: | OpenAIRE |
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