Corneal Hydration Monitored by Laser-induced Breakdown Spectroscopy
| Autor: | Harilaos Ginis, G Kounis, Ioannis G. Pallikaris, Theodore G. Papazoglou, Demetrios Anglos, Leonidas P. Naoumidis |
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| Rok vydání: | 1998 |
| Předmět: |
Models
Anatomic Materials science genetic structures medicine.medical_treatment Analytical chemistry In Vitro Techniques Excimer Photorefractive Keratectomy Absorption law.invention Cornea Optics Body Water law Monitoring Intraoperative medicine Animals Humans Irradiation Laser-induced breakdown spectroscopy business.industry Spectrum Analysis Atomic emission spectroscopy Pulse duration Laser eye diseases Photorefractive keratectomy Ophthalmology medicine.anatomical_structure Methacrylates Lasers Excimer Surgery Rabbits sense organs business |
| Zdroj: | Scopus-Elsevier ResearcherID |
| ISSN: | 1081-597X |
| DOI: | 10.3928/1081-597x-19981101-16 |
| Popis: | BACKGROUND: Corneal hydration is an important factor in laser corneal ablation. In photorefractive keratectomy (PRK), corneal ablation rate and final ablation surface quality are strongly dependent on corneal hydration. We used, a spectroscopic technique for monitoring corneal hydration during PRK. METHODS: Hydroxyethylmethacrylate (HEMA) was employed for corneal hydration modeling. Hydrated HEMA samples were irradiated with a pulsed Nd:YAG laser (1064 run, 10 mJ/pulse, pulse duration 15 nsec). Successive emission spectra corresponding to different degrees of hydration were recorded on a gated optical multichannel analyzer. The weight of the sample and hence its water content was monitored during the entire procedure with a sensitive balance. One rabbit and one human cornea were used to demonstrate the spectral analogy between the model and corneal tissue. RESULTS: The most noticeable dependence on water content of the substrate was that of atomic emission lines of Ca at 393 nm and 396 nm. CONCLUSION: Plasma emission spectra exhibited significant dependence on sample hydration. This dependence can be used for estimation of water content of irradiated model material and real cornea. [J Refract Surg 1998;14:655-660] |
| Databáze: | OpenAIRE |
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