Controllable molecular motors engineered from myosin and RNA
| Autor: | Laura Y. Kim, Clarence Yu Cheng, Paul V. Ruijgrok, Rhiju Das, Gregory M. Alushin, Zev Bryant, Tosan Omabegho, Pinar S. Gurel |
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| Rok vydání: | 2017 |
| Předmět: |
Models
Molecular 0301 basic medicine business.product_category Materials science Swine Oligonucleotides Biophysics Biomedical Engineering Nanotechnology Bioengineering 02 engineering and technology Myosins Biology Ribosome Article Motion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Myosin Molecular motor Animals General Materials Science Electrical and Electronic Engineering Actin 030304 developmental biology 0303 health sciences Lever Base Sequence Oligonucleotide RNA Processivity 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics 030104 developmental biology chemistry Nucleic acid Nucleic Acid Conformation Biological system 0210 nano-technology business 030217 neurology & neurosurgery DNA |
| Zdroj: | Nature nanotechnology |
| ISSN: | 1748-3395 1748-3387 |
| Popis: | Engineering biomolecular motors can provide direct tests of structure-function relationships and customized components for controlling molecular transport in artificial systems or in living cells. In previous work, synthetic nucleic acid motors have been designed from first principles and engineered for versatile programmable control; in a complementary approach, natural protein motors have been modified to combine some control and tunability with favorable evolved properties such as speed, processivity, and biological compatibility. Nucleoprotein motors such as the ribosome illustrate the potential for tightly integrating protein and nucleic acid components to enable sophisticated functionality. However, this potential has only begun to be explored in pioneering work harnessing DNA scaffolds to dictate the spacing, number, and composition of tethered protein motors. Here, we describe myosin motors that incorporate RNA lever arms, forming hybrid assemblies in which conformational changes in the protein motor domain are amplified and redirected by nucleic acid structures. The RNA lever arm geometry determines the speed and direction of motor transport, and can be dynamically controlled using programmed transitions in lever arm structure. We have characterized the hybrid motors using in vitro assays of propelled actin filaments, single-molecule tracking of tetrameric processive walkers, cryoelectron microscopy, and MOHCA-seq structural probing. Our results confirm the operation of motors designed to reversibly change direction in response to oligonucleotide signals that drive strand-displacement reactions. This work enables future applications in which complex transport systems are programmed using sequence-addressable control of speed and direction in heterogeneous motor collections; in principle, the signaling inputs can originate from transcriptional circuits or DNA computations. Similar combinations of nucleic acid structures and diverse protein motors may provide a general strategy for controlling dynamic behavior, with collective motor properties further determined by precise arrangement and orientation on larger nucleic acid scaffolds. |
| Databáze: | OpenAIRE |
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