Landscape of Hopx expression in cells of the immune system
| Autor: | Adeleye Opejin, Cindy Gross, Jessica Bourque, Rajan Jain, Jonathan A. Epstein, Daniel Hawiger, Courtney A. Iberg, Alexey Surnov |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
H1-99
Science (General) Multidisciplinary medicine.diagnostic_test Cellular differentiation Immune cells Regulator Expression Biology Hopx Steady state Flow cytometry Cell biology Social sciences (General) Mice Q1-390 Immune system scRNA-seq medicine Homeobox Stem cell CD8 Function (biology) Research Article |
| Zdroj: | Heliyon, Vol 7, Iss 11, Pp e08311-(2021) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Homeodomain only protein (Hopx) is a regulator of cell differentiation and function, and it has also emerged as a crucial marker of specific developmental and differentiation potentials. Hopx expression and functions have been identified in some stem cells, tumors, and in certain immune cells. However, expression of Hopx in immune cells remains insufficiently characterized. Here we report a comprehensive pattern of Hopx expression in multiple types of immune cells under steady state conditions. By utilizing single-cell RNA sequencing (scRNA-seq) and flow cytometric analysis, we characterize a constitutive expression of Hopx in specific subsets of CD4+ and CD8+ T cells and B cells, as well as natural killer (NK), NKT, and myeloid cells. In contrast, Hopx expression is not present in conventional dendritic cells and eosinophils. The utility of identifying expression of Hopx in immune cells may prove vital in delineating specific roles of Hopx under multiple immune conditions. Hopx; Immune cells; scRNA-seq; Mice; Flow cytometry; Expression; Steady state. |
| Databáze: | OpenAIRE |
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