Screening genes associated with elevated neutrophil‑to‑lymphocyte ratio in chronic heart failure

Autor: Lan Cheng, Lihua Ji, Guo-xing Wan, Wenbin Xia, Yonggang Zhang
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Oncology
Cancer Research
Neutrophils
Interleukin 1 receptor
type II
030204 cardiovascular system & hematology
Biochemistry
Leukocyte Count
0302 clinical medicine
Databases
Genetic
Protein Interaction Mapping
Gene Regulatory Networks
Receptors
Interleukin-1 Type II
Lymphocytes
Annexin A3
Symporters
Articles
Middle Aged
Protein-Tyrosine Kinases
chronic heart failure
Molecular Medicine
Female
medicine.medical_specialty
Organic Cation Transport Proteins
GPI-Linked Proteins
Amidohydrolases
Neutrophil mediated immunity
03 medical and health sciences
Internal medicine
Genetics
medicine
Humans
Neutrophil to lymphocyte ratio
neutrophil to lymphocyte ratio
Molecular Biology
Aged
Heart Failure
Receptors
Interleukin-1 Type I
Receiver operating characteristic
business.industry
fungi
Cancer
Molecular Sequence Annotation
medicine.disease
Molecular medicine
differentially expressed gene
Solute carrier family
Gene Ontology
030104 developmental biology
Gene Expression Regulation
Heart failure
Chronic Disease
business
Cell Adhesion Molecules
Biomarkers
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2018.9132
Popis: Neutrophil-to-lymphocyte ratio (NLR) is commonly considered a useful prognostic index for many cardiovascular diseases; however, it has limited sensitivity and specificity. Factors associated with elevated NLR may aid in the prediction of prognosis with heart failure (HF) in combination with NLR. The present study sought to identify decisive factors associated with NLR in HF patients and investigate their association with elevated NLR. The gene expression profile for blood samples from 197 individuals with chronic heart failure (CHF), with corresponding hematological parameters and clinical data were obtained from the public database, {"type":"entrez-geo","attrs":{"text":"GSE77343","term_id":"77343"}}GSE77343. Differentially expressed genes (DEGs) were identified, and Gene Ontology and pathway enrichment analyses were performed. The protein-protein interaction network was constructed with the Search Tool for the Retrieval of Interacting Genes along with Cytoscape. Receiver operating characteristic curves for predictive power, sensitivity and specificity were constructed. The present study identified specific associated DEGs by using Pearson linear correlation and logistic regression analysis. A mean NLR of 3.96 was determined as the cutoff value in the analysis. In total, 31 genes were initially identified as DEGs associated with elevated NLR. They were mainly enriched in neutrophil activation and neutrophil mediated immunity, in fluid shear stress and atherosclerosis, and transcriptional misregulation in cancer. Three focused DEGs, solute carrier family 22 member 4 (SLC22A4), interleukin-1 receptor 2 (IL1R2) and vanin 3 (VNN3), were finally revealed to be independently associated with elevated NLR in CHF patients. The present study demonstrated that the three genes SLC22A4, IL1R2 and VNN3 may be independently associated with elevated NLR in CHF patients as potential decisive factors of NLR.
Databáze: OpenAIRE
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