Indefinite growth of mammalian cell autotrophs on amino acids, vitamins, and glucose
| Autor: | Régine Mariage-Samson, Jana Hillova, Miroslav Hill |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Keratinocytes
Cell Mitosis CHO Cells Culture Media Serum-Free Chinese hamster Cricetinae medicine Animals Humans Autotroph Amino Acids chemistry.chemical_classification biology Cell growth Chinese hamster ovary cell Vitamins Cell Biology Carbohydrate biology.organism_classification Clone Cells Amino acid Glucose medicine.anatomical_structure Biochemistry chemistry Salts |
| Zdroj: | Cell Biology International Reports. 15:899-906 |
| ISSN: | 0309-1651 |
| DOI: | 10.1016/0309-1651(91)90140-e |
| Popis: | Established mammalian cells segregate variants, named autotrophs, able to proliferate in the absence of hormones and proteins of any kind. We provided complete formulas for autotrophic media composed of amino acids, vitamins, glucose, and inorganic salts only and showed that such media are sufficient to sustain continuous propagation of Chinese hamster fibroblasts and human keratinocytes. The cells grew on bare polystyrene and divided every 32 to 44 hours reaching approximately 8 x 10(5) cells/cm2. The results showed that the autotrophs may replace conventional cultures in most experimental and industrial cell production systems. |
| Databáze: | OpenAIRE |
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