A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model
| Autor: | Eun-Hye Lee, Cheol-Heui Yun, Seung Hyun Han, Mi-Ran Cho, Ok-Jin Park, Jaehong Hwang, Moon-Hee Jeong, Sun-Young Kim, Seungryong Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Halomonas stevensii Firmicutes In vivo micro-computed tomography Microbiology Article 03 medical and health sciences 0302 clinical medicine stomatognathic system Virology medicine lcsh:QH301-705.5 Periodontitis Halomonas biology Microbiota Bacteroidetes 030206 dentistry apical periodontitis Rat apical periodontitis model medicine.disease biology.organism_classification stomatognathic diseases 030104 developmental biology lcsh:Biology (General) Pulp (tooth) bacteria Proteobacteria Bradyrhizobium japonicum |
| Zdroj: | Microorganisms, Vol 8, Iss 1174, p 1174 (2020) Microorganisms Volume 8 Issue 8 |
| ISSN: | 2076-2607 |
| Popis: | Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level, Escherichia, Streptococcus, Lactobacillus, Rodentibacter, and Bacteroidetes were dominant genera in the pulp chamber. The most abundant genera in the root apex were Bradyrhizobium, Halomonas, and Escherichia. The species Azospirillum oryzae increased in the pulp chamber, whereas the species Bradyrhizobium japonicum and Halomonas stevensii were highly observed in the root apex as the disease progressed. The experimental rat model of apical periodontitis demonstrated a relationship between the microbiota and the apical periodontitis progression. |
| Databáze: | OpenAIRE |
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