Oxidation of isomaltose, gentiobiose, and melibiose by membrane-bound quinoprotein glucose dehydrogenase from acetic acid bacteria
| Autor: | Hiromi Murakami, Taro Kiso, Hirofumi Sato, Takaaki Kiryu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Disaccharides Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Gentiobiose Acetic acid bacteria Melibiose Molecular Biology Quinoprotein glucose dehydrogenase Acetic Acid Chromatography Bacteria biology Organic Chemistry Glucose 1-Dehydrogenase General Medicine Maltose Isomaltose biology.organism_classification Thin-layer chromatography Lactobionic acid 030104 developmental biology chemistry Oxidation-Reduction Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 84:507-517 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2019.1689095 |
| Popis: | Membrane-bound quinoprotein glucose dehydrogenase from acetic acid bacteria produces lactobionic acid by the oxidation of lactose. Its enzymatic activity on lactose and maltose is much lower than that on d-glucose. For that reason, the activity of the enzyme on disaccharides has been considered low. In this study, we show that the isomaltose-oxidizing activity of acetic acid bacteria is much higher than their lactose-oxidizing activity. In addition to isomaltose, the enzyme oxidized gentiobiose and melibiose to the same extent. According to the characteristics of the isomaltose-oxidizing activity and investigations using dehydrogenase-deficient mutant bacteria, we identified the responsible enzyme as membrane-bound quinoprotein glucose dehydrogenase. Abbreviations: AAB: acetic acid bacteria; m-GDH: membrane-bound quinoprotein glucose dehydrogenase; DCIP: 2,6-dichlorophenolindophenol; DP: degree of polymerization; HPAEC-PAD: high-performance anion-exchange chromatography with pulsed amperometric detection; NMR: nuclear magnetic resonance; TLC: thin layer chromatography; COSY: correlation spectroscopy |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|