3D bio-etching of a complex composite-like embryonic tissue
| Autor: | Melis Hazar, Jiho Song, Philip R. LeDuc, William C. Messner, YongTae Kim, Lance A. Davidson |
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| Rok vydání: | 2015 |
| Předmět: |
Cell signaling
Embryo Nonmammalian Cell Biomedical Engineering Morphogenesis Xenopus Bioengineering Biology Biochemistry Article Xenopus laevis Imaging Three-Dimensional Tissue engineering medicine Animals Tissue Engineering Regeneration (biology) Sodium Dodecyl Sulfate General Chemistry Embryonic Tissue Micro-Electrical-Mechanical Systems Microfluidic Analytical Techniques biology.organism_classification Multicellular organism medicine.anatomical_structure Biophysics Biomedical engineering |
| Zdroj: | Lab on a Chip. 15:3293-3299 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | Morphogenesis involves a complex series of cell signaling, migration and differentiation events that are coordinated as tissues self-assemble during embryonic development. Collective cell movements such as those that occur during morphogenesis have typically been studied in 2D with single layers of cultured cells adhering to rigid substrates such as glass or plastic. In vivo, the intricacies of the 3D microenvironment and complex 3D responses are pivotal in the formation of functional tissues. To study such processes as collective cell movements within 3D multilayered tissues, we developed a microfluidic technique capable of producing complex 3D laminar multicellular structures. We call this technique "3D tissue-etching" because it is analogous to techniques used in the microelectromechanics (MEMS) field where complex 3D structures are built by successively removing material from a monolithic solid through subtractive manufacturing. We use a custom-designed microfluidic control system to deliver a range of tissue etching reagents (detergents, chelators, proteases, etc.) to specific regions of multilayered tissues. These tissues were previously isolated by microsurgical excision from embryos of the African claw-toed frog, Xenopus laevis. The ability to shape the 3D form of multicellular tissues and to control 3D stimulation will have a high impact on tissue engineering and regeneration applications in bioengineering and medicine as well as provide significant improvements in the synthesis of highly complex 3D integrated multicellular biosystems. |
| Databáze: | OpenAIRE |
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