Electrophoretic separation of S. pombe chromosomes in polyacrylamide solutions using a constant field
| Autor: | Tadeusz Guszczynski, Andreas Chrambach |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Chromatography
Chemistry Polyacrylamide Biophysics Analytical chemistry Cell Biology Constant field Biochemistry Matrix (chemical analysis) Molecular Weight chemistry.chemical_compound Electrophoresis Capillary electrophoresis Electric field Schizosaccharomyces Agarose Electrophoresis Polyacrylamide Gel Indicators and Reagents Chromosomes Fungal DNA Fungal Molecular Biology DNA |
| Zdroj: | Biochemical and biophysical research communications. 179(1) |
| ISSN: | 0006-291X |
| Popis: | Previous electrophoretic separations of megabase (Mb) sized DNA have been achieved in pulsed electric fields, using agarose gel as a matrix. The present study demonstrates separations of Mb sized DNA due to a retardation of migration in proportion to the concentration of uncrosslinked polyacrylamide of 5 x 10(6) molecular weight, using a constant electric field. Potentially, the method should be applicable to large DNA in general, greatly reducing the instrumental complexity of such separations and rendering them compatible with capillary electrophoresis apparatus. |
| Databáze: | OpenAIRE |
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