Molecular Identification of Prune Dwarf Virus (PDV) Infecting Sweet Cherry in Canada and Development of a PDV Full-Length Infectious cDNA Clone
| Autor: | Jonathan S. Griffiths, Aaron J. Simkovich, Yinzi Li, Aiming Wang, Susanne E. Kohalmi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
clone (Java method) DNA Complementary fruit tree virus Nicotiana benthamiana Prune dwarf virus Genome Viral Prunus avium 01 natural sciences Microbiology Article Virus 03 medical and health sciences Virology Plant Diseases 030304 developmental biology Ontario Ilarvirus Infectivity next generation sequencing 0303 health sciences Base Sequence biology infectious clone High-Throughput Nucleotide Sequencing food and beverages biology.organism_classification QR1-502 Dwarfing RNA silencing Infectious Diseases RNA Viral ilarvirus Prunus 010606 plant biology & botany |
| Zdroj: | Viruses Volume 13 Issue 10 Viruses, Vol 13, Iss 2025, p 2025 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13102025 |
| Popis: | Prune dwarf virus (PDV) is a member of ilarviruses that infects stone fruit species such as cherry, plum and peach, and ornamentally grown trees worldwide. The virus lacks an RNA silencing suppressor. Infection by PDV either alone, or its mixed infection with other viruses causes deteriorated fruit marketability and reduced fruit yields. Here, we report the molecular identification of PDV from sweet cherry in the prominent fruit growing region of Ontario, Canada known as the Niagara fruit belt using next generation sequencing of small interfering RNAs (siRNAs). We assessed its incidence in an experimental farm and determined the full genome sequence of this PDV isolate. We further constructed an infectious cDNA clone. Inoculation of the natural host cherry with this clone induced a dwarfing phenotype. We also examined its infectivity on several common experimental hosts. We found that it was infectious on cucurbits (cucumber and squash) with clear symptoms and Nicotiana benthamiana without causing noticeable symptoms, and it was unable to infect Arabidopsis thaliana. As generating infectious clones for woody plants is very challenging with limited success, the PDV infectious clone developed from this study will be a useful tool to facilitate molecular studies on PDV and related Prunus-infecting viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|