Antibacterial metabolite prospecting from Actinomycetes isolated from waste damped soils from Thika, central part of Kenya
| Autor: | Gatebe Erastus, Naomi Maina, Abebe Bizuye, Christine Bii |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Bacteria pathogens lcsh:RC955-962 Metabolite lcsh:R 030106 microbiology lcsh:Medicine Bioactive isolates Biology 03 medical and health sciences chemistry.chemical_compound Infectious Diseases chemistry Environmental chemistry Soil water Prospecting Waste damped soil Thika Antibacterial activity screening |
| Zdroj: | Asian Pacific Journal of Tropical Disease, Vol 7, Iss 12, Pp 757-764 (2017) |
| ISSN: | 2222-1808 |
| DOI: | 10.12980/apjtd.7.2017d7-162 |
| Popis: | Objective: To evaluate antibacterial activity of the metabolites produced by actinomycetes isolates isolated from waste damped soil in Thika. Methods: Soil samples were collected randomly from selected waste damping sites and composite soil samples were prepared. Composite soil samples were pre-treated with dry heat and CaCO3. 0.1 mL of soil suspension from 10-5 serial diluted composite soil sample spread on selective media for selective growth and isolation. The primary and secondary screenings and evaluation of antibacterial active isolates were done by streak plate and well diffusion assay, respectively. Results: 29 (23.2%) isolates showed antibacterial activity during primary screening. From these, isolate KGT22 showed 30 ± 0 mm, 31.3 ± 0.6 mm, 30 ± 0 mm and 36 ± 1 mm inhibition zone against E. coli ATCC 25922, S. boydii, S. typhi and V. cholerea, respectively. Isolate KDO24 showed antibacterial activity against both MRSA (16.25 ± 0.50 mm) and E. coli (26.5 ± 0.58 mm). Supernatants from 11 (37.93%) isolates showed antibacterial activity during secondary screening. Supernatant from BML45, KGT31 and PLS 34 showed better inhibition zone (17 ± 1 mm) against E. coli ATCC25922, S. boydii and S. typhi, respectively. Conclusions: Therefore, antibacterial activity result showed that soils collected from Thika waste damping sites are potential sources of antibacterial producing isolates. |
| Databáze: | OpenAIRE |
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