Degradation of Gadd45 mRNA by nonsense-mediated decay is essential for viability
| Autor: | Alex Chapin, Jonathan O. Nelson, Julie Hollien, Mark M. Metzstein, Kristin A. Moore |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
gadd45 QH301-705.5 Science Nonsense-mediated decay MRNA Decay Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences mRNA decay NMD Biology (General) Gene Cell survival Gene knockdown Messenger RNA General Immunology and Microbiology Gadd45 General Neuroscience upf1 General Medicine drosophila Molecular biology 030104 developmental biology upf2 Medicine Function (biology) |
| Zdroj: | eLife, Vol 5 (2016) |
| ISSN: | 2050-084X |
| Popis: | The nonsense-mediated mRNA decay (NMD) pathway functions to degrade both abnormal and wild-type mRNAs. NMD is essential for viability in most organisms, but the molecular basis for this requirement is unknown. Here we show that a single, conserved NMD target, the mRNA coding for the stress response factor growth arrest and DNA-damage inducible 45 (GADD45) can account for lethality in Drosophila lacking core NMD genes. Moreover, depletion of Gadd45 in mammalian cells rescues the cell survival defects associated with NMD knockdown. Our findings demonstrate that degradation of Gadd45 mRNA is the essential NMD function and, surprisingly, that the surveillance of abnormal mRNAs by this pathway is not necessarily required for viability. |
| Databáze: | OpenAIRE |
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