Nonintegrating Knockdown and Customized Scaffold Design Enhances Human Adipose-Derived Stem Cells in Skeletal Repair
Autor: | Derrick C. Wan, Benjamin Levi, Shuli Li, Jeong S. Hyun, Joseph C. Wu, Min Lee, Emily R. Nelson, Daniel T. Montoro, Natalina Quarto, Aaron W. James, Jason C. Glotzbach, Fang Jun Jia, Michael T. Longaker, Mei Huang, Geoffrey C. Gurtner |
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Rok vydání: | 2011 |
Předmět: |
Adult
Male Bone Regeneration Time Factors Cellular differentiation Genetic Vectors Bone Morphogenetic Protein 2 Mice Nude Mice Transgenic Biology Bone morphogenetic protein Bone morphogenetic protein 2 Regenerative medicine Article Mice Young Adult Implants Experimental Polylactic Acid-Polyglycolic Acid Copolymer Tissue engineering Osteogenesis Animals Humans Lactic Acid RNA Small Interfering Noggin Bone regeneration Tissue Engineering Tissue Scaffolds Stem Cells Lentivirus Skull Cell Differentiation Cell Biology Middle Aged Molecular biology Recombinant Proteins Cell biology Adipose Tissue Gene Knockdown Techniques Molecular Medicine Female Stem cell Carrier Proteins Polyglycolic Acid Signal Transduction Developmental Biology |
Zdroj: | Stem Cells. 29:2018-2029 |
ISSN: | 1549-4918 1066-5099 |
Popis: | An urgent need exists in clinical medicine for suitable alternatives to available techniques for bone tissue repair. Human adipose-derived stem cells (hASCs) represent a readily available, autogenous cell source with well-documented in vivo osteogenic potential. In this article, we manipulated Noggin expression levels in hASCs using lentiviral and nonintegrating minicircle short hairpin ribonucleic acid (shRNA) methodologies in vitro and in vivo to enhance hASC osteogenesis. Human ASCs with Noggin knockdown showed significantly increased bone morphogenetic protein (BMP) signaling and osteogenic differentiation both in vitro and in vivo, and when placed onto a BMP-releasing scaffold embedded with lentiviral Noggin shRNA particles, hASCs more rapidly healed mouse calvarial defects. This study therefore suggests that genetic targeting of hASCs combined with custom scaffold design can optimize hASCs for skeletal regenerative medicine. |
Databáze: | OpenAIRE |
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