Rapid Multimethod for Verification and Determination of Toxic Pesticides in Whole Blood by Means of Capillary GC-MS
| Autor: | Michael Uihlein, Thomas Frenzel, Heinz Sochor, Karl Speer |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chemical Health and Safety
Chromatography Pesticide residue Chemistry Health Toxicology and Mutagenesis Forensic Medicine Pesticide Toxicology Mass spectrometry Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Analytical Chemistry Lethal Dose 50 Environmental chemistry Standard addition Humans Environmental Chemistry Gas chromatography Pesticides Gas chromatography–mass spectrometry Quantitative analysis (chemistry) Whole blood |
| Zdroj: | Journal of Analytical Toxicology. 24:365-371 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/24.5.365 |
| Popis: | A rapid and single multimethod was developed to determine substances of different pesticide classes in whole blood in the event of acute human intoxications, as required by EU Commission Directive 96/46. The method was validated by an in-house and an independent laboratory validation. Whole blood is hemolyzed and then deproteinized. After extraction of the supernatant, blood levels are determined by gas chromatography-mass spectrometry. The method, which can be performed within 120 rain, covers 15 active substances (8 organophosphate pesticides, 2 carbamates, 3 pyrethroids, 1 azole, and I organochlorine pesticide) classified as toxic or very toxic. These compounds can be identified down to concentrations between 100 and 1000 ng/mL by comparison of their mass spectra to those in a commercial pesticide mass spectra library. Using the standard addition method, they can be quantitated down to concentrations between 30 and 200 ng/mL. These limits of quantitation are considered to be sufficient in comparison to respective LDs0 values. |
| Databáze: | OpenAIRE |
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