The Role of Epithelial Mesenchymal Transition Markers in Thyroid Carcinoma Progression
| Autor: | Heather Hardin, Sofia Asioli, Darya Buehler, Celina Montemayor-Garcia, Zhenying Guo, Herbert Chen, Ricardo V. Lloyd, Carolina Larrain |
|---|---|
| Přispěvatelé: | Montemayor-Garcia C, Hardin H, Guo Z, Larrain C, Buehler D, Asioli S, Chen H, Lloyd RV |
| Rok vydání: | 2013 |
| Předmět: |
Adenoma
Adult Male endocrine system Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Adolescent endocrine system diseases Endocrinology Diabetes and Metabolism Thyroid Gland Biology Article Cell Line Pathology and Forensic Medicine Thyroid carcinoma Endocrinology Adenocarcinoma Follicular Biomarkers Tumor medicine Carcinoma Humans ZEB1 Thyroid Neoplasms Epithelial–mesenchymal transition Thyroid cancer Aged Aged 80 and over Homeodomain Proteins E-Cadherin Tissue microarray Smad7 Thyroid anaplastic thyroid carcinoma Zinc Finger E-box-Binding Homeobox 1 General Medicine Middle Aged Cadherins medicine.disease Carcinoma Papillary medicine.anatomical_structure Tumor progression Disease Progression Adenocarcinoma Female Transcription Factors |
| Zdroj: | Endocrine Pathology. 24:206-212 |
| ISSN: | 1559-0097 1046-3976 |
| DOI: | 10.1007/s12022-013-9272-9 |
| Popis: | Understanding the molecular mechanisms involved in thyroid cancer progression may provide targets for more effective treatment of aggressive thyroid cancers. Epithelial-mesenchymal transition (EMT) is a major pathologic mechanism in tumor progression and is linked to the acquisition of stem-like properties of cancer cells. We examined expression of ZEB1 which activates EMT by binding to the E-box elements in the E-cadherin promoter, and expression of E-cadherin in normal and neoplastic thyroid tissues in a tissue microarray (TMA) which included 127 neoplasms and 10 normal thyroid specimens. Thyroid follicular adenomas (FA, n=32), follicular thyroid carcinomas (FTC, n=28), and papillary thyroid carcinomas (PTC, n=57) all expressed E-cadherin and were mostly negative for ZEB1 while most anaplastic thyroid carcinomas (ATC, n=10) were negative for E-cadherin, but positive for ZEB1. A validation set of 10 whole sections of ATCs showed 90% of cases positive for ZEB1 and all cases were negative for E-cadherin. Analysis of three cell lines (normal thyroid, NTHY-OR13-1; PTC, TPC-1 and ATC, THJ-21T) showed that the ATC cell line expressed the highest levels of ZEB1 while the normal thyroid cell line expressed the highest levels of E-Cadherin. Quantitative RT-PCR analyses showed that Smad7 mRNA was significantly higher in ATC than in any other group (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink