Fluorescence in situ hybridization reanalysis of day-6 human blastocysts diagnosed with aneuploidy on day 3
| Autor: | David L. Hill, Catherine Marin DeUgarte, Man Li, Alan H. DeCherney, Mark Surrey, H. Danzer |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Aneuploidy Biology Preimplantation genetic diagnosis Intracytoplasmic sperm injection medicine Humans Blastocyst In Situ Hybridization Fluorescence Preimplantation Diagnosis Retrospective Studies Gynecology In vitro fertilisation medicine.diagnostic_test Reproducibility of Results Obstetrics and Gynecology Embryo Blastomere medicine.disease medicine.anatomical_structure Reproductive Medicine embryonic structures Female Fluorescence in situ hybridization |
| Zdroj: | Fertility and Sterility. 84:1395-1400 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2005.04.068 |
| Popis: | Objective To determine the concordance of day-6 blastocyst analysis with the day-3 fluorescence in situ hybridization (FISH) aneuploidy diagnosis. Design Retrospective study. Setting In vitro fertilization laboratory. Patient(s) Six hundred sixty embryos were included from 94 IVF/intracytoplasmic sperm injection patients undergoing preimplantation genetic diagnosis. Intervention(s) None. Main Outcome Measure(s) Single blastomeres biopsied on day 3. Aneuploidy screening for chromosomes 13, 18, 21, X, and Y were analyzed. Left-over blastocysts were reanalyzed on day 6. Result(s) Among the 660 embryos evaluated, 367 (55.6%) were euploid and 281 (42.6%) were aneuploid. Of the euploid embryos, 213 embryos were transferred, 68 were frozen on day 5, and 86 were left. All 281 aneuploid embryos were further cultured, and 55 (19.6%) progressed to blastocysts. When FISH reanalysis was performed, 33 of 55 blastocysts (60%) were confirmed aneuploid in concordance with the day-3 diagnosis. However, 22 of 55 blastocysts (40%) were determined to be euploid. In addition, 207 aneuploid embryos (73.7%) arrested before day 6, as opposed to 32 of the 86 euploid embryos (37.2%). Conclusion(s) Day-3 single-cell embryo biopsy reveals that aneuploidy can be confirmed in 60.7% of the blastocysts on reanalysis. The majority of discordance is most likely due to embryo mosaicism and possibly a limited ability to "self-correct." |
| Databáze: | OpenAIRE |
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