Daunorubicin Inhibits Gene Expression of Cyclooxygenase-2 in Vascular Smooth Muscle Cells
| Autor: | Eizo Kakishita, Ichiro Wakabayashi, Kenichi Yasui, Munetaka Negoro, Akira Suehiro |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Vascular smooth muscle
Daunorubicin Gene Expression Prostacyclin Biology Nitric Oxide Muscle Smooth Vascular Nitric oxide chemistry.chemical_compound Gene expression polycyclic compounds medicine Animals Aorta Messenger RNA Antibiotics Antineoplastic Dose-Response Relationship Drug NF-kappa B Hematology Epoprostenol Molecular biology Rats Isoenzymes carbohydrates (lipids) chemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases biology.protein Arachidonic acid Cyclooxygenase Interleukin-1 medicine.drug |
| Zdroj: | Thrombosis Research. 103:233-240 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/s0049-3848(01)00324-3 |
| Popis: | Daunorubicin (0.1-1 microM) concentration-dependently inhibited prostacyclin production induced by interleukin-1beta (IL-1beta, 2.5 ng/ml) in cultured aortic smooth muscle cells isolated from rats. IL-1beta stimulation caused activation of nuclear factor-kappaB (NF-kappaB) and expression of cyclooxygenase-2 (COX-2) mRNA and protein, which were inhibited by daunorubicin. However, COX activity, evaluated by conversion of exogenous arachidonic acid to prostacyclin, was not affected by daunorubicin (0.1-1 microM). Protein expression of COX-1 and NF-kappaB was not affected by daunorubicin. Daunorubicin also inhibited nitric oxide (NO) production induced by IL-1beta. These results suggest that daunorubicin attenuated prostacyclin synthesis through inhibiting expression of COX-2 mRNA, which could be explained by perturbation of NF-kappaB activation. |
| Databáze: | OpenAIRE |
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