Double knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice
| Autor: | Masahiro Sakamoto, Keishi Osakabe, Takuji Miyamoto, Yuki Tobimatsu, Yuriko Osakabe, Rie Takada, Toshiaki Umezawa, Masaomi Yamamura, Shiro Suzuki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Magnetic Resonance Spectroscopy Mutant Lignocellulosic biomass Plant Science Biology Lignin complex mixtures 01 natural sciences Cell wall Gene Knockout Techniques 03 medical and health sciences chemistry.chemical_compound Cell Wall CRISPR-Associated Protein 9 Genetics Gene Phylogeny Plant Proteins Gene Editing Oryza sativa Plant Stems Mutagenesis food and beverages Oryza General Medicine Plants Genetically Modified WRKY protein domain 030104 developmental biology Biochemistry chemistry CRISPR-Cas Systems Agronomy and Crop Science Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Science. 296 |
| ISSN: | 1873-2259 |
| Popis: | Breeding to enrich lignin, a major component of lignocelluloses, in plants contributes to enhanced applications of lignocellulosic biomass into solid biofuels and valuable aromatic chemicals. To collect information on enhancing lignin deposition in grass species, important lignocellulose feedstocks, we generated rice (Oryza sativa) transgenic lines deficient in OsWRKY36 and OsWRKY102, which encode putative transcriptional repressors for secondary cell wall formation. We used CRISPR/Cas9-mediated targeted mutagenesis and closely characterized their altered cell walls using chemical and nuclear magnetic resonance (NMR) methods. Both OsWRKY36 and OsWRKY102 mutations significantly increased lignin content by up to 28 % and 32 %, respectively. Additionally, OsWRKY36/OsWRKY102-double-mutant lines displayed lignin enrichment of cell walls (by up to 41 %) with substantially altered culm morphology over the single-mutant lines as well as the wild-type controls. Our chemical and NMR analyses showed that relative abundances of guaiacyl and p-coumarate units were slightly higher and lower, respectively, in the WRKY mutant lignins compared with those in the wild-type lignins. Our results provide evidence that both OsWRKY36 and OsWRKY102 are associated with repression of rice lignification. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect