Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III

Autor:	Ernesto Cesar Pinto Leal-Junior, Paulo de Tarso Camillo de Carvalho, Andreia Aparecida Da Silva, Caroline Sobral de Melo Rambo, Rodolfo de Paula Vieira, Solange Almeida dos Santos, Ana Carolina Araruna Alves
Rok vydání:	2013
Předmět:	Male medicine.medical_specialty medicine.medical_treatment Gallium Inflammation Dermatology Matrix metalloproteinase Real-Time Polymerase Chain Reaction Indium Collagen Type I Diabetes Mellitus Experimental Collagen Type III Diabetes mellitus Internal medicine Animals Medicine RNA Messenger Low-Level Light Therapy Rats Wistar Low level laser therapy Skin Wound Healing business.industry Streptozotocin medicine.disease Rats Surgery Endocrinology Real-time polymerase chain reaction Matrix Metalloproteinase 9 Matrix Metalloproteinase 2 Lasers Semiconductor medicine.symptom business Wound healing Aluminum medicine.drug
Zdroj:	Journal of Cosmetic and Laser Therapy. 15:210-216
ISSN:	1476-4180 1476-4172
DOI:	10.3109/14764172.2012.761345
Popis:	The present study aimed to determine if LLLT restores the balance between mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9) and also the balance between collagen types I and III during the healing process of diabetic wounds. One hundred and twenty male Wistar rats were distributed in Control (untreated non-diabetic rats: UND); Laser (laser treated in non-diabetic rats: LTND); Diabetic (diabetic rats non-laser treated rats: UD); and Diabetic+ Laser (diabetic rats laser treated: DLT) groups. The diabetes model using streptozotocin efficiently induced diabetes, as demonstrated through increased levels of blood glucose. Diode laser (50 mW, 660 nm, 4 J/cm(2), 80 s) was applied a single time after scare induction. Twenty-four hours after LLLT application, rats were euthanized, the scarred areas were collected for MMP-2 and MMP-9 mRNA analysis and also for histological analysis (inflammation and types I and III collagen). The results demonstrated that scare in untreated diabetic rats significantly increased the MMP-2 and MMP-9 expression compared with that in non-diabetic rats (p < 0.05), while LLLT significantly reduced MMP-2 and MMP-9 expression compared with that in untreated diabetic rats (p < 0.05). To conclude, the results also showed that LLLT was able to alter the expression of MMP-9 as well as accelerate the production of collagen and increase the total percentage of collagen type III in diabetic animals.
Databáze:	OpenAIRE
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