Antibody‐Dependent Cellular Cytotoxic Activity of Cells in the Rat Metrial Gland in Pregnancy
| Autor: | I. J. Stewart, S. Peel |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Male medicine.medical_specialty Immunology Cell Fluorescent Antibody Technique chemical and pharmacologic phenomena Spleen Metrial Gland Antigens CD Pregnancy Internal medicine Tumor Cells Cultured medicine Animals Cytotoxic T cell Rats Wistar Cytotoxicity Antibody-dependent cell-mediated cytotoxicity biology Effector Macrophages Antibody-Dependent Cell Cytotoxicity Histocompatibility Antigens Class II hemic and immune systems General Medicine Molecular biology Rats medicine.anatomical_structure Endocrinology biology.protein Pregnancy Animal Female Peritoneum Antibody |
| Zdroj: | Scandinavian Journal of Immunology. 43:16-22 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1046/j.1365-3083.1996.d01-6.x |
| Popis: | Antibody-dependent cellular cytotoxic (ADCC) activity of cells in rat metrial glands at days 13 and 20 of pregnancy has been examined. A 51chromium-release cytotoxicity assay was carried out using Yac-1 cells and P815 cells as targets in the presence of the corresponding, heat inactivated rat antibody or non-immune rat serum. No killing was observed when effector metrial gland cells and target cells were cultured in the presence of non-immune rat serum. In the presence of rat antibody to Yac-1 cells, or rat antibody to P815 cells, cells from the metrial glands of rats killed at day 13 of pregnancy demonstrated ADCC activity, but cells from the metrial glands of rats killed at day 20 of pregnancy showed no ADCC activity. Spleen and peritoneal exudate cells displayed ADCC activity. Immunohistological and immunocytological studies showed OX-6, OX-34, OX-35 and OX-42 positive cells were present at days 13 and 20 of pregnancy, in sections of metrial glands and in the cell suspensions prepared for the cytotoxicity assays. It is suggested that macrophages are the cells most likely to be responsible for the ADCC activity in the rat metrial gland. |
| Databáze: | OpenAIRE |
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