Natural Splicing of Exon 2 of Human Interleukin-15 Receptor α-Chain mRNA Results in a Shortened Form with a Distinct Pattern of Expression
| Autor: | Olivier Boisteau, Yannick Jacques, Sylvie Raher, Jérôme Bernard, Sigrid Dubois, Sabine Leroy, Stephane Minvielle, Philippe Lehours, Anne Godard, Florence Magrangeas |
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| Rok vydání: | 1999 |
| Předmět: |
Glycosylation
RNA Splicing Biology Polymerase Chain Reaction Biochemistry Cell Line Exon medicine Animals Humans RNA Messenger Nuclear membrane Cytokine binding Receptor Molecular Biology Gene Interleukin-15 Receptors Interleukin-15 Receptors Interleukin-2 Exons U937 Cells Cell Biology Molecular biology Interleukin-15 receptor medicine.anatomical_structure Gene Expression Regulation COS Cells RNA splicing Nuclear localization sequence |
| Zdroj: | Journal of Biological Chemistry. 274:26978-26984 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.274.38.26978 |
| Popis: | We report the existence of eight different interleukin-15 receptor alpha-chain (IL-15Ralpha) transcripts resulting from exon-splicing mechanisms within the IL-15Ralpha gene. Two main classes of transcripts can be distinguished that do or do not (Delta2 isoforms) contain the exon 2-coding sequence. Both classes were expressed in numerous cell lines and tissues (including peripheral blood lymphocytes) at comparable levels and could be transcribed in COS-7 cells, and the proteins were expressed at the cell surface. Both receptor forms displayed numerous glycosylation states, reflecting differential usage of a single N-glycosylation site as well as extensive O-glycosylations. Whereas IL-15Ralpha bound IL-15 with high affinity, Delta2IL-15Ralpha was unable to bind IL-15, thus revealing the indispensable role of the exon 2-encoded domain in cytokine binding. A large proportion of IL-15Ralpha was expressed at the nuclear membrane with some intranuclear localization, supporting a potential direct action of the IL-15.IL-15Ralpha complex at the nuclear level. In sharp contrast, Delta2IL-15Ralpha was found only in the non-nuclear membrane compartments, indicating that the exon 2-encoded domain (which is shown to contain a potential nuclear localization signal) plays an important role in receptor post-translational routing. Together, our data indicate that exon 2 splicing of human IL-15Ralpha is a natural process that might play regulatory roles at different levels. |
| Databáze: | OpenAIRE |
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