Estrogenic effects of Ficus umbellata Vahl. (Moraceae) extracts and their ability to alleviate some menopausal symptoms induced by ovariectomy in Wistar rats
| Autor: | Sefirin Djiogue, Thomas Michel, Nde Chantal Beatrice Magne, Colin Clyne, Charline Florence Awounfack, Stéphane Zingue, Jules Tchatchou, Tânia Beatriz Creczynski-Pasa, André Monchot, Evelyn Winter, Dieudonné Njamen, Xavier Fernandez |
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| Rok vydání: | 2016 |
| Předmět: |
Cell Survival
Ovariectomy Estrogen receptor Epithelium 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Drug Discovery Animals Humans Medicine Umbelliferones Rats Wistar Mammary Glands Human Cells Cultured Pharmacology 030219 obstetrics & reproductive medicine biology Traditional medicine Plant Extracts business.industry Uterus Proteins Estrogens Organ Size Ficus Moraceae biology.organism_classification medicine.disease Coumarin In vitro Rats Menopause Receptors Estrogen chemistry Hot Flashes Vagina Ovariectomized rat Female Phytoestrogens business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Ethnopharmacology. 179:332-344 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Ficus umbellata Vahl. (Moraceae) is a medicinal plant used in Cameroon to treat amenorrhea as well as other physiological disorders related to menopause. Aim of study In order to justify scientifically its traditional use, the estrogen-like properties of the aqueous (AE) and methanol (MeOH) extracts of F. umbellata were investigated. Material and methods In vitro , the ability of different extracts of F. umbellata to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney (HEK293T) cells transfected with ERs was tested. In vivo , a 3-day uterotrophic assay and the capacity of the extracts to alleviate hot flushes in ovariectomized adult rats were tested. Using a bioassay-guided fractionation the major compound of F. umbellata was isolated and tested in vitro on HEK293T-ERα and ERβ cells. Results AE and MeOH extracts significantly altered ERα as well as ERβ activities. In vivo , both extracts significantly increase the uterine and vaginal epithelium thickness, and uterine total protein levels in a dose dependent manner. Interestingly, both extracts of F. umbellata at the dose of 100 mg/kg BW significantly decreased the total number, average duration as well as frequency of hot flushes in experimental rats compared to age-matched OVX controls. Finally, 7-methylumbelliferone, a coumarin was characterized as the major compound of F. umbellata ; however this compound did not transactivate ERα as well ERβ in vitro . Conclusion These aforementioned results suggest that F. umbellata extracts as used by the traditional practitioner have estrogen-like effects and may alleviate some menopausal problems such as vaginal dryness and hot flushes. |
| Databáze: | OpenAIRE |
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