Habituation and inhibitory avoidance training alter brain regional levels of benzodiazepine-like molecules and are affected by intracerebral flumazenil microinjection
| Autor: | Haydee Viola, Claudio Da Cunha, C. Wolfman, Jorge H. Medina, Ivan Izquierdo, Diana Jerusalinsky, Miguelina Levi de Stein |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Flumazenil
Male medicine.medical_specialty Microinjections medicine.drug_class Central nervous system Hippocampus Flunitrazepam Amygdala Benzodiazepines Reference Values Internal medicine Muscarinic acetylcholine receptor Avoidance Learning medicine Animals Habituation Habituation Psychophysiologic Molecular Biology Brain Chemistry Chemistry General Neuroscience Brain Rats Inbred Strains Receptors GABA-A Receptor antagonist Receptors Muscarinic Rats Quinuclidinyl Benzilate medicine.anatomical_structure Endocrinology Cerebral cortex Anesthesia Neurology (clinical) Stereotyped Behavior Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 548:74-80 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(91)91108-d |
| Popis: | The effects of habituation and inhibitory avoidance training on the rat brain regional levels of benzodiazepine (BZD)-like molecules and on central type BZD binding sites were examined. BZD-like immunoreactivity was decreased by 26–50% in the amygdala, cerebral cortex and septum of rats sacrificed immediately after stepping-down from the platform of an inhibitory avoidance apparatus (non-trained group) as compared to naive controls. Rats submitted to a second step-down session 20 h later (habituated group) have significantly lower BZD-like immunoreactivity in the septum (−60%) as compared to non-trained animals. Rats exposed to an inhibitory avoidance training, i.e. stepping-down and receiving a footshock (trained group), showed a significant reduction in the content of BZD-like molecules in cerebral cortex (−44%), amygdala (−68%), septum (−80%) and hippocampus (−82%) as compared to non-trained rats. In addition, the density of central type BZD binding sites was slightly increased in the hippocampus and septum of trained rats. No changes were observed in the apparent dissociation constant. No changes were observed in parallel measurements of [3H]-l-quinuclidinyl benzylate binding constants at cholinergic muscarinic binding sites. The immediate posttraining intrahippocampal bilateral injection of the central type BZD receptor antagonist flumazenil (10 nmol/hippocampus), enhanced the retention of habituation but not when injected in the amygdala or septum. In contrast, retention of the inhibitory avoidance task was significantly increased by flumazenil administered bilaterally into any of the 3 brain structures. Taken together, the present findings strongly support the hypothesis that posttraining storage processes are normally down-regulated by endogenous BZD-like molecules and suggest a differential regional involvement of this modulatory mechanism in memory, according to the task. |
| Databáze: | OpenAIRE |
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