Inhibition of dendritic L-Type calcium current by memantine in frog tectum
| Autor: | Antanas Kuras, Artūras Grigaliūnas, Armantas Baginskas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Calcium Channels
L-Type Rana temporaria Neurotransmission Receptors N-Methyl-D-Aspartate Rana Antiparkinson Agents Retina Physiology Retinal ganglion cells Dendrites Calcium channels L-Type Synaptic transmission Memantine medicine Animals Tectum Mesencephali 612.822 [udc] Voltage-dependent calcium channel business.industry General Medicine Calcium Channel Blockers frog tectum dendritic L-type calcium current memantine Alzheimer’s disease medicine.anatomical_structure Retinal ganglion cell Biophysics NMDA receptor sense organs Tectum business Neuroscience medicine.drug |
| Zdroj: | Medicina, Kaunas : Lietuvos sveikatos mokslų universitetas, 2013, t. 49, Nr. 9, p. 409-414 Medicina; Volume 49; Issue 9; Pages: 64 |
| ISSN: | 1010-660X |
| Popis: | The aim of the study was to explore the effects of memantine on responses elicited in the frog tectum by the bursts of spikes of moderate strength of a single retina ganglion cell and to gain an insight about the effect of memantine on the L-type Ca2+ current. Material and Methods. The experiments were performed in vivo on adult frogs (Rana temporaria). An individual retina ganglion cell (or its retinotectal fiber) was stimulated by current pulses delivered through a multichannel stimulating electrode positioned on the retina. Responses to the discharge of a single retinal ganglion cell were recorded in the tectum by an extracellular carbonfiber microelectrode positioned in the terminal arborization of the retinotectal fiber in the tectum layer F. The solution of memantine (1-amino-3,5-dimethyladamantane) hydrochloride (30 or 45 μM) was applied onto the surface of the tectum by perfusion at a rate of 0.4 mL/min. Results. Memantine (30–45 μM) largely inhibited the L-type Ca2+ channel-mediated slow negative wave and late discharges seen in the tectum responses without any effect on fast synaptic retinotectal transmission. Conclusions. Our results suggest that the neuroprotective effect of memantine could arise not only through the inhibition of the NMDA receptor current but also through the suppression of the L-type Ca2+ current. |
| Databáze: | OpenAIRE |
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