Red light accelerates the formation of a human dermal equivalent
| Autor: | Ana Mg Plepis, Cláudia Bernal, Anna Cb Oliveira, P. F. C. Menezes, Thayz F.L. Morais, Janice Rodrigues Perussi, Virginia Ca Martins |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Light Swine Cell Biomedical Engineering Biocompatible Materials 02 engineering and technology Absorption (skin) Biomaterials Extracellular matrix 03 medical and health sciences Tissue engineering Chlorocebus aethiops medicine Animals Humans Vero Cells Cells Cultured Skin Artificial Tissue Engineering Tissue Scaffolds Chemistry Cell growth Biomaterial Dermis 021001 nanoscience & nanotechnology CÉLULAS 030104 developmental biology medicine.anatomical_structure Cell culture Biophysics Collagen 0210 nano-technology Porosity Type I collagen |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1530-8022 |
| Popis: | Development of biomaterials' substitutes and/or equivalents to mimic normal tissue is a current challenge in tissue engineering. Thus, three-dimensional cell culture using type I collagen as a polymeric matrix cell support designed to promote cell proliferation and differentiation was employed to create a dermal equivalent in vitro, as well to evaluate the photobiomodulation using red light. Polymeric matrix cell support was prepared from porcine serous collagen (1.1%) hydrolyzed for 96 h. The biomaterial exhibited porosity of 95%, a median pore of 44 µm and channels with an average distance between the walls of 78 ± 14 µm. The absorption of culture medium was 95%, and the sponge showed no cytotoxicity to Vero cells, a non-tumor cell line. Additionally, it was observed that irradiation with light at 630 nm (fluency 30 J cm-2) leads to the cellular photobiomodulation in both monolayer and human dermal equivalent (three-dimensional cell culture system). It was also verified that the cells cultured in the presence of the polymeric matrix cell support, allows differentiation and extracellular matrix secretion. Therefore, the results showed that the collagen sponge used as polymeric matrix cell support and the photobiomodulation at 630 nm are efficient for the production of a reconstructed human dermal equivalent in vitro. |
| Databáze: | OpenAIRE |
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