AdcAII of Streptococcus pneumoniae Affects Pneumococcal Invasiveness
| Autor: | Nicholas C. Fitzkee, Adam N. Cassella, Beth Mann, Steven M. Gunnell, Elaine Tuomanen, Lance E. Keller, Lisa A. Scherkenbach, Justin A. Thornton, Jason W. Rosch, Matthew Brown, Rebecca A Hill, Lindsey R. Brown |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Mutant lcsh:Medicine Plasma protein binding medicine.disease_cause Pneumococcal Infections Microbiology Pathogenesis Mice 03 medical and health sciences Bacterial Proteins Laminin Cell Line Tumor Nasopharynx Streptococcus pneumoniae Cell Adhesion medicine Animals Humans lcsh:Science Cation Transport Proteins Oligonucleotide Array Sequence Analysis Mutation Multidisciplinary biology lcsh:R Wild type Gene Expression Regulation Bacterial medicine.disease Extracellular Matrix Mice Inbred C57BL Zinc Pneumococcal infections 030104 developmental biology Mutagenesis biology.protein ATP-Binding Cassette Transporters Female lcsh:Q Research Article Protein Binding |
| Zdroj: | PLoS ONE, Vol 11, Iss 1, p e0146785 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Across bacterial species, metal binding proteins can serve functions in pathogenesis in addition to regulating metal homeostasis. We have compared and contrasted the activities of zinc (Zn2+)-binding lipoproteins AdcA and AdcAII in the Streptococcus pneumoniae TIGR4 background. Exposure to Zn2+-limiting conditions resulted in delayed growth in a strain lacking AdcAII (ΔAdcAII) when compared to wild type bacteria or a mutant lacking AdcA (ΔAdcA). AdcAII failed to interact with the extracellular matrix protein laminin despite homology to laminin-binding proteins of related streptococci. Deletion of AdcA or AdcAII led to significantly increased invasion of A549 human lung epithelial cells and a trend toward increased invasion in vivo. Loss of AdcAII, but not AdcA, was shown to negatively impact early colonization of the nasopharynx. Our findings suggest that expression of AdcAII affects invasiveness of S. pneumoniae in response to available Zn2+ concentrations. |
| Databáze: | OpenAIRE |
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