Hematopoietic Stem Cell Subtypes Expand Differentially during Development and Display Distinct Lymphopoietic Programs
| Autor: | Keegan Rowe, Nima Aghaeepour, Claudia Benz, Stefan Wöhrer, Adrian Cortes, David G. Kent, Heidi Mader, Christopher Day, Elaine Ma, David Q. Treloar, Connie J. Eaves, Ryan R. Brinkman, Michael R. Copley |
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| Rok vydání: | 2012 |
| Předmět: |
Cellular differentiation
Biology Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Pregnancy Genetics medicine Animals Humans Cell Lineage Lymphopoiesis Progenitor cell Cellular Senescence 030304 developmental biology 0303 health sciences medicine.diagnostic_test Hematopoietic stem cell Cell Differentiation Cell Biology Flow Cytometry Hematopoietic Stem Cells Cell biology Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Molecular Medicine Female Bone marrow Stem cell |
| Zdroj: | Cell Stem Cell. 10:273-283 |
| ISSN: | 1934-5909 |
| DOI: | 10.1016/j.stem.2012.02.007 |
| Popis: | SummaryAdult hematopoietic stem cells (HSCs) with serially transplantable activity comprise two subtypes. One shows a balanced output of mature lymphoid and myeloid cells; the other appears selectively lymphoid deficient. We now show that both of these HSC subtypes are present in the fetal liver (at a 1:10 ratio) with the rarer, lymphoid-deficient HSCs immediately gaining an increased representation in the fetal bone marrow, suggesting that the marrow niche plays a key role in regulating their ensuing preferential amplification. Clonal analysis of HSC expansion posttransplant showed that both subtypes display an extensive but variable self-renewal activity with occasional interconversion. Clonal analysis of their differentiation programs demonstrated functional and molecular as well as quantitative HSC subtype-specific differences in the lymphoid progenitors they generate but an indistinguishable production of multipotent and myeloid-restricted progenitors. These findings establish a level of heterogeneity in HSC differentiation and expansion control that may have relevance to stem cell populations in other hierarchically organized tissues. |
| Databáze: | OpenAIRE |
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