| Autor: |
Tingting Xie, Guangya Wang, Spencer C. Ding, Wing-Shan Lee, Suk Hang Cheng, Rebecca W. Y. Chan, Ze Zhou, Mary-Jane L. Ma, Diana S. C. Han, Jeremy Y. C. Teoh, W. K. Jacky Lam, Peiyong Jiang, Rossa W. K. Chiu, K. C. Allen Chan, Y. M. Dennis Lo |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
NPJ genomic medicine. 7(1) |
| ISSN: |
2056-7944 |
| Popis: |
Single-stranded ends of double-stranded DNA (jagged ends) are more abundant in urinary DNA than in plasma DNA. However, the lengths of jagged ends in urinary DNA remained undetermined, as a previous method used for urinary DNA jagged end sequencing analysis (Jag-seq) relied on unmethylation at CpG sites, limiting the resolution. Here, we performed high-resolution Jag-seq analysis using methylation at non-CpG cytosine sites, allowing determination of exact length of jagged ends. The urinary DNA bore longer jagged ends (~26-nt) than plasma DNA (~17-nt). The jagged end length distribution displayed 10-nt periodicities in urinary DNA, which were much less observable in plasma DNA. Amplitude of the 10-nt periodicities increased in patients with renal cell carcinoma. Heparin treatment of urine diminished the 10-nt periodicities. The urinary DNA jagged ends often extended into nucleosomal cores, suggesting potential interactions with histones. This study has thus advanced our knowledge of jagged ends in urine DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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