Impact of healthy aging on active bacterial assemblages throughout the gastrointestinal tract
| Autor: | Riccardo Vasapolli, Alexander Link, Peter Malfertheiner, Dirk Schomburg, Frederike Palm, Anke Lux, Christian Schulz, Ramiro Vilchez-Vargas, Robert Geffers, Bianca Simon, Kerstin Schütte, Dietmar H. Pieper |
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| Přispěvatelé: | HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Aging Saliva Physiology microbiome RC799-869 Gut flora Microbiology Asymptomatic Feces Intestinal mucosa RNA Ribosomal 16S medicine Humans Prospective Studies Microbiome Intestinal Mucosa Aged Aged 80 and over Gastrointestinal tract Bacteria Host Microbial Interactions biology gut microbiota Probiotics Gastroenterology Middle Aged Anthropometry Diseases of the digestive system. Gastroenterology biology.organism_classification Gastrointestinal Microbiome Infectious Diseases Healthy aging nutrition healthy aging physical fitness Female medicine.symptom Research Article Research Paper |
| Zdroj: | Gut Microbes, Vol 13, Iss 1 (2021) Gut microbes United States Gut Microbes article-version (VoR) Version of Record |
| ISSN: | 1949-0984 1949-0976 |
| Popis: | The adaption of gut microbiota (GM) throughout human life is a key factor in maintaining health. Interventions to restore a healthy GM composition may have the potential to improve health and disease outcomes in the elderly. We performed a comprehensive characterization of changes in the luminal and mucosa-associated microbiota composition in elderly compared with younger healthy individuals. Samples from saliva and feces, and biopsies from the upper and lower gastrointestinal tract (UGIT, LGIT), were collected from 59 asymptomatic individuals grouped by age: 40–55, 56–70, and 71–85 years). All underwent anthropometric, geriatric, and nutritional assessment. RNA was extracted and reverse-transcribed into complementary DNA; the V1–V2 regions of 16S ribosomal RNA genes were amplified and sequenced. Abundances of the taxa in all taxonomic ranks in each sample type were used to construct sample-similarity matrices by the Bray–Curtis algorithm. Significant differences between defined groups were assessed by analysis of similarity. The bacterial community showed strong interindividual variations and a clear distinction between samples from UGIT, LGIT, and feces. While in saliva some taxa were affected by aging, this number was considerably greater in UGIT and was subsequently higher in LGIT. Unexpectedly, aging scarcely influenced the bacterial community of feces over the age range of 40–85 years. The development of interventions to preserve and restore human health with increased age by establishing a healthy gut microbiome should not rely solely on data from fecal analysis, as the intestinal mucosa is affected by more significant changes, which differ from those observed in fecal analyses. |
| Databáze: | OpenAIRE |
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