Coupling a bio-accumulator organism and MALDI-TOF MS: an early warning detection system for microcystins in water bodies

Autor: Francesca Caviglia, Giuseppe Torzillo, Valentin Pflueger, Andrea Lami, Lyudmila Kamburska, Giuseppe Morabito, Mauro Tonolla, Nicoletta Riccardi, Ana Margarita Silva Benavides, Rosaria Lauceri, Martina Austoni
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of applied phycology (Dordr., Online) (2017). doi:10.1007/s10811-017-1193-4
info:cnr-pdr/source/autori:Rosaria Lauceri (1), Martina Austoni (1), Francesca Caviglia(2), Lyudmila Kamburska(1), Andrea Lami(1), Giuseppe Morabito(1), Valentin Pflueger(3), Ana Margarita Silva Benavides(4), Mauro Tonolla(5), Giuseppe Torzillo(6), Nicoletta Riccardi(1)/titolo:Coupling a bio-accumulator organism and MALDI-TOF MS: an early warning detection system for microcystins in water bodies./doi:10.1007%2Fs10811-017-1193-4/rivista:Journal of applied phycology (Dordr., Online)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume
riUAL. Repositorio Institucional de la Universidad de Almería
Universidad de Almería
Journal of Applied Phycology, vol.29, pp.2979–2988
Kérwá
Universidad de Costa Rica
instacron:UCR
Journal of Applied Phycology
DOI: 10.1007/s10811-017-1193-4
Popis: Microcystins produced by freshwater cyanobacteria pose serious threats to human health and are a growing problem in drinking water supplies worldwide. Toxin detection and identification in water reservoirs when cyanobacterial density is still low is a key step to predict harmful algal blooms and to allow the safe use of the resource. For this purpose, developing sensitive and rapid methods of analysis is crucial. Adopting a strategy that couples the use of a filter-feeder organism (Unio elongatulus, Pfeiffer 1825 (Bivalvia: Unionidae)) with matrix-assisted laser desorption ionization—time of flight mass spectrometry (MALDI-TOF MS) technology, effective microcystin detection was achieved. The performances of MALDI-TOF MS, high-liquid-performance chromatography with diode array detector (HPLC-DAD) and enzyme-linked immunosorbent assays (ELISA) test were compared. The results obtained in this study suggest that the exploitation of an efficient bio-accumulator organism, coupled with a rapid and sensitive analytical method, can be a very useful strategy in monitoring programs for early and prompt risk management. UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencias del Mar y Limnología (CIMAR)
Databáze: OpenAIRE