Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro
| Autor: | Hiroharu Kawahara, Yuichi Inoue, Hirofumi Tachibana, Akira Iwamoto |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
food.ingredient Pectin Clinical Biochemistry Size-exclusion chromatography Biomedical Engineering Bioengineering Immunoglobulin E Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine food Oral administration medicine Pectinase biology Chemistry Cell Biology Atopic dermatitis JAACT Issue medicine.disease Molecular biology In vitro 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Biotechnology |
| Zdroj: | Cytotechnology. 71:573-581 |
| ISSN: | 1573-0778 0920-9069 |
| Popis: | We found that strawberry (Fragaria x ananassa) extract has an IgE production suppressive activity and its oral administration improved skin manifestation in atopic dermatitis model mice. In present study, we identified an active substance using the IgE-producing human myeloma cell line U266. Gel filtration experiment indicated that the IgE suppressor was more than 6 kDa in molecular size. In addition, its pectinase treatment inhibited the activity, suggesting that the active substance in strawberry extract is pectin. Among solutions of water-(WP), hexametaphosphate-(HXP), acid-(HP) and alkali soluble pectin (OHP) extracted from strawberry, only OHP suppressed IgE production, and their suppressive activity was cancelled by pectinase treatment. In addition, OHP extracted from apple also inhibited IgE production. Furthermore, OHP also suppressed IgE production and did not affect IgG and IgM production in human peripheral blood mononuclear cells in an in vitro immunization condition. From these results, we concluded that OHP was an IgE suppressor in strawberry extract. |
| Databáze: | OpenAIRE |
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