Identification and tissue expression profiling of candidate UDP-glycosyltransferase genes expressed in Holotrichia parallela motschulsky antennae
Autor: | Jinxin Wang, Jun Wang, Jiankun Yi, Jing-Jiang Zhou, Yu Pan, Shuang Yang, Xu Zhang, Shang Wang, Yu Liu, Jinghui Xi |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Olfactory system Arthropod Antennae Male Glycosylation media_common.quotation_subject Olfaction Insect digestive system 01 natural sciences Uridine Diphosphate 03 medical and health sciences chemistry.chemical_compound Animals Amino Acid Sequence Spodoptera littoralis Gene Phylogeny media_common Phylogenetic tree biology Gene Expression Profiling Glycosyltransferases General Medicine biology.organism_classification Coleoptera 010602 entomology 030104 developmental biology Biochemistry chemistry Insect Science Insect Proteins Female Drosophila melanogaster Agronomy and Crop Science Sequence Alignment |
Zdroj: | Bulletin of entomological research. 108(6) |
ISSN: | 1475-2670 |
Popis: | It is difficult to control Holotrichia parallela Motschulsky with chemical insecticides due to the larvae's soil-living habit, thus the pest has caused great economic losses in agriculture. In addition, uridine diphosphate-glycosyltransferases (UGTs) catalyze the glycosylation process of a variety of small lipophilic molecules with sugars to produce water-soluble glycosides, and play multiple roles in detoxification, endobiotic modulation, and sequestration in an insect. Some UGTs were found specifically expressed in antennae of Drosophila melanogaster and Spodoptera littoralis, and glucurono-conjugated odorants could not elicit any olfactory signals, suggesting that the UGTs may play roles in odorant inactivation by biotransformation. In the current study, we performed a genome-wide analysis of the candidate UGT family in the dark black chafer, H. parallela. Based on a UGT gene signature and the similarity of these genes to UGT homologs from other organisms, 20 putative H. parallela UGT genes were identified. Bioinformatics analysis was used to predict sequence and structural features of H. parallela UGT proteins, and revealed important domains and residues involved in sugar donor binding and catalysis by comparison with human UGT2B7. Phylogenetic analysis of these 20 UGT protein sequences revealed eight major groups, including both order-specific and conserved groups, which are common to more than one order. Of these 20 UGT genes, HparUGT1265-1, HparUGT3119, and HparUGT8312 were highly (>100-fold change) expressed in antennae, suggesting a possible role in olfactory tissue, and most likely in odorant inactivation and olfactory processing. The remaining UGT genes were expressed in all tissues (head, thorax, abdomen, leg, and wing), indicating that these UGTs likely have different biological functions. This study provides the fundamental basis for determining the function of UGTs in a highly specialized olfactory organ, the H. parallela antenna. |
Databáze: | OpenAIRE |
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