Identification of a Novel Single Nucleotide Polymorphism in Porcine Beta-Defensin-1 Gene
| Autor: | R. T. Venkatachalapathy, A. P. Usha, D. R. Pruthviraj |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Large White Yorkshire 040301 veterinary sciences Population lcsh:Animal biochemistry Single-nucleotide polymorphism Locus (genetics) Biology Article law.invention 0403 veterinary science 03 medical and health sciences law Genotype Ankamali education Genotyping Gene Selection lcsh:QP501-801 Polymerase chain reaction Disease Resistance lcsh:SF1-1100 Genetics education.field_of_study Antimicrobial Peptide Single-strand conformation polymorphism 04 agricultural and veterinary sciences Molecular biology 030104 developmental biology Polymerase Chain Reaction-Single Strand Conformation Polymorphism [PCR-SSCP] Animal Science and Zoology lcsh:Animal culture Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 29, Iss 3, Pp 315-320 (2016) Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity. |
| Databáze: | OpenAIRE |
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