Human Cytomegalovirus Inhibits Autophagy of Renal Tubular Epithelial Cells and Promotes Cellular Enlargement
| Autor: | Ana C. López Giuliani, Eva Hernández, María J. Tohmé, Clémence Taisne, Julieta S. Roldán, Clara García Samartino, Marion Lussignol, Patrice Codogno, María I. Colombo, Audrey Esclatine, Laura R. Delgui |
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| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Virulence et Latence des Herpesvirus (HERPES), Département Virologie (Dpt Viro), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE13-0015,Autophagy,Contôle chimique de l'autophagie(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Human cytomegalovirus POLARIZATION viruses [SDV]Life Sciences [q-bio] lcsh:QR1-502 Cytomegalovirus lcsh:Microbiology purl.org/becyt/ford/1 [https] renal cells Cellular and Infection Microbiology Cells Cultured Original Research Cytopathic effect Kidney Cilium virus diseases Primary Cilium PRIMARY CILIUM 3. Good health Cell biology Cytomegaly HUMAN CYTOMEGALOVIRUS Infectious Diseases medicine.anatomical_structure cellular size CELLULAR SIZE Cytomegalovirus Infections Human Cytomegalovirus AUTOPHAGY CYTOMEGALY purl.org/becyt/ford/3 [https] RENAL CELLS Microbiology (medical) 030106 microbiology Immunology Biology Microbiology purl.org/becyt/ford/3.3 [https] 03 medical and health sciences Ciliogenesis medicine Autophagy Humans purl.org/becyt/ford/1.6 [https] Tropism polarization CYTOPATHIC EFFECT Epithelial Cells medicine.disease 030104 developmental biology Homeostasis |
| Zdroj: | Frontiers in Cellular and Infection Microbiology Frontiers in Cellular and Infection Microbiology, 2020, 10, pp.474. ⟨10.3389/fcimb.2020.00474⟩ Frontiers in Cellular and Infection Microbiology, Vol 10 (2020) Frontiers in Cellular and Infection Microbiology, Frontiers, 2020, 10, pp.474. ⟨10.3389/fcimb.2020.00474⟩ CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2235-2988 |
| Popis: | Human Cytomegalovirus (HCMV) is a frequent opportunistic pathogen in immunosuppressed patients, which can be involved in kidney allograft dysfunction and rejection. In order to study the pathophysiology of HCMV renal diseases, we concentrated on the impact of HCMV infection on human renal tubular epithelial HK-2 cells. Our aim was to develop a model of infection of HK-2 cells by using the viral strain TB40/E, that contains the extended cell tropism of clinical isolates and the efficient viral multiplication in cell culture of laboratory-adapted strains. We observed that HK-2 cells can be infected by HCMV and expressed viral antigens, but they do not produce extracellular viral particles. We then studied the interplay of HCMV with ciliogenesis and autophagy. Primary cilium (PC) is a stress sensor important to maintain renal tissue homeostasis that projects from the apical side into the lumen of tubule cells. PC formation and length were not modified by HCMV infection. Autophagy, another stress response process critically required for normal kidney functions, was inhibited by HCMV in HK-2 cells with a reduction in the autophagic flux. HCMV classically induces an enlargement of infected cells in vivo and in vitro, and we observed that HCMV infection led to an enlargement of the HK-2 cell volume. Our results constitute therefore an excellent starting point to further explore the role of these mechanisms in renal cells dysfunction. Fil: López Giuliani, Ana C.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hernández, Eva. Centre D'etudes de Saclay; Francia Fil: Tohmé Chapini, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Taisne, Clémence. Centre D'etudes de Saclay; Francia Fil: Roldan, Julieta Suyay. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: García Samartino, Clara. Universidad Nacional de Cuyo; Argentina Fil: Lussignol, Marion. Centre D'etudes de Saclay; Francia Fil: Codogno, Patrice. Sorbonne University; Francia Fil: Colombo, María Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Esclatine, Audrey. Centre D'etudes de Saclay; Francia Fil: Delgui, Laura Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina |
| Databáze: | OpenAIRE |
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