Expression of alpha-hemoglobin stabilizing protein gene during human erythropoiesis
| Autor: | Camila Oresco dos Santos, Fernando Ferreira Costa, Adriana S. S. Duarte, Sara Teresinha Olalla Saad |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Cellular differentiation Biology Adenosine Triphosphate Gene expression Genetics medicine Humans Erythropoiesis Molecular Biology Cells Cultured Regulation of gene expression Cell Differentiation Cell Biology Hematology Blood Proteins Molecular biology Globins Red blood cell Real-time polymerase chain reaction medicine.anatomical_structure Gene Expression Regulation Erythropoietin Hemoglobin medicine.drug Molecular Chaperones |
| Zdroj: | Experimental hematology. 32(2) |
| ISSN: | 0301-472X |
| Popis: | alpha-Hemoglobin stabilizing protein (AHSP) is an abundant, erythroid-specific protein that forms a stable complex with free alpha-hemoglobin but not with beta-hemoglobin or hemoglobin A. As such, AHSP is required for normal erythropoiesis, probably acting by blocking the deleterious effects of free alpha-hemoglobin precipitation. In order to study the levels of expression of the AHSP gene during the different phases of erythropoiesis, we carried out a two-phase liquid culture of erythroid cells and real-time quantitative polymerase chain reaction. Blood from control volunteers was cultured with erythropoietin to stimulate differentiation. The different stages of erythropoiesis were confirmed by morphologic and flow cytometric analysis. The results showed a progressive increase in AHSP gene expression following the expression of alpha-globin gene, during maturation of the red blood cell precursors, confirming the probable important function of this protein during normal erythropoiesis. |
| Databáze: | OpenAIRE |
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