Sphingosine 1-phosphate induces Ca2+ transients and cytoskeletal rearrangement in C2C12 myoblastic cells
| Autor: | Bruno Tiribilli, Elisabetta Meacci, Lucia Formigli, Franco Quercioli, Massimo Vassalli, Sandra Zecchi Orlandini, Claudia Piperio, Chiara Bencini, Fabio Francini, Daniele Nosi, Paola Bruni |
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| Rok vydání: | 2002 |
| Předmět: |
Intracellular Fluid
Calcium Channels L-Type Physiology Inositol 1 4 5-Trisphosphate Suramin Biology Calcium in biology Cell Line Diglycerides Mice chemistry.chemical_compound NF-KappaB Inhibitor alpha Sphingosine Caffeine Extracellular Animals Calcium Signaling Sphingosine-1-phosphate Muscle Skeletal Cytoskeleton Fluorescent Dyes Aniline Compounds Microscopy Confocal Phospholipase D Ryanodine Receptor Calcium Release Channel Cell Biology Calcium Channel Blockers Cell biology DNA-Binding Proteins Cytosol Receptors Lysophospholipid Xanthenes chemistry Biochemistry Potassium Calcium I-kappa B Proteins PROTEIN KINASE C SKELETAL MUSCLE FIBERS LYSOPHOSPHATIDIC ACID SMOOTH MUSCLE SIGNAL TRANSDUCTION CYSTIC FIBROSIS PHOSPHOLIPASE D RHO KINASE INTRACELLULAR CALCIUM MEDIATED ACTIVATION Lysophospholipids Extracellular Space Intracellular Muscle Contraction Signal Transduction |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00378.2001 |
| Popis: | In many cell systems, sphingosine 1-phosphate (SPP) increases cytosolic Ca2+concentration ([Ca2+]i) by acting as intracellular mediator and extracellular ligand. We recently demonstrated (Meacci E, Cencetti F, Formigli L, Squecco R, Donati C, Tiribilli B, Quercioli F, Zecchi-Orlandini S, Francini F, and Bruni P. Biochem J 362: 349–357, 2002) involvement of endothelial differentiation gene (Edg) receptors (Rs) specific for SPP in agonist-mediated Ca2+ response of a mouse skeletal myoblastic (C2C12) cell line. Here, we investigated the Ca2+ sources of SPP-mediated Ca2+ transients in C2C12 cells and the possible correlation of ion response to cytoskeletal rearrangement. Confocal fluorescence imaging of C2C12 cells preloaded with Ca2+ dye fluo 3 revealed that SPP elicited a transient Ca2+ increase propagating as a wave throughout the cell. This response required extracellular and intracellular Ca2+ pool mobilization. Indeed, it was significantly reduced by removal of external Ca2+, pretreatment with nifedipine (blocker of L-type plasma membrane Ca2+channels), and inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]-mediated Ca2+pathway inhibitors. Involvement of EdgRs was tested with suramin (specific inhibitor of Edg-3). Fluorescence associated with Ins(1,4,5)P3Rs and L-type Ca2+channels was evident in C2C12 cells. SPP also induced C2C12 cell contraction. This event, however, was unrelated to [Ca2+]i increase, because the two phenomena were temporally shifted. We propose that SPP may promote C2C12 cell contraction through Ca2+-independent mechanisms. |
| Databáze: | OpenAIRE |
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